JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (1): 85-91.doi: 10.6040/j.issn.1671-7554.0.2016.575

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Safety limit of cold extracorporeal perfusion and ischaemic storage of human uterus

XU Xinxin1, LIU Wen1, WAN Jipeng2, LI Tao2, WANG Fei2, WANG Xia3, YAN Lei1, LI Changzhong2, CHEN Zijiang1   

  1. 1. Center for Reproductive Medicine, Shandong University;
    National Research Center for Assisted Reproductive Technology and Reproductive Genetics;
    Key Laboratory of Reproductive Endocrinology(Shandong University), Ministry of Education;
    Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250001, Shandong, China;
    2. Department of Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    3. Institute of Physiology, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2016-05-19 Online:2017-01-10 Published:2017-01-10

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Abstract: Objective To investigate the safety limit of cold extracorporeal perfusion with histidine-tryptophane-ketoglutarate(HTK)and ischaemic storage of human uterus. Methods A total of 7 uteri were obtained from patients undergoing extensive hysterectomy for cervical carcinoma. Three of them were perfused with cold HTK solution through each artery to detect the safe height of perfusion and to establish the mathematical model showing the relation between perfusion height and pressure. The other four uteri were perused at proper height, and then endometrium and myometrial tissues were collected and stored in HTK or normal saline(NS). The samples were then divided into 0 h group, HTK 3 h 山 东 大 学 学 报 (医 学 版)55卷1期 -许鑫鑫,等.人离体子宫冷灌注及冷缺血保存的安全区间 \=-group, HTK 6 h group, HTK 24 h group, NS 3 h group, NS 6 h group and NS 24 h group. The cellular morphology and contractility of each group was detected, and the tolerability of human uterine tissue to cold ischaemic after short-time perfusion was determined. Results The corresponding perfusion height of 90-140 mmHg perfusion pressure was 62-122 cm. Compared with 0 h group, the HTK 3 h and HTK 6 h groups did not exhibit any major changes, while the HTK 24 h group and NS groups displayed degenerative changes such as hydropic changes, loss of intercellular contacts, hydropic mitochondria and coarse chromatin. No apparent difference in contractility was observed in specimens in HTK 3 h, HTK 6 h and HTK 24 h groups(P=0.772), but the contractility weakened significantly in the NS groups compared with the HTK groups(P<0.05). Conclusion The safe height is 62-122 cm for extracorporeal perfusion of human uterus by cold HTK solution; human uterine myometrial tissue can tolerate cold ischaemia for at least 6 h when stored in HTK solution after short-time extracorporeal perfusion.

Key words: Extracorporeal perfusion, HTK solution, Human uterine transplantation, Cold ischaemic storage, Organ preservation

CLC Number: 

  • R617
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[1] LI Jindong, WANG Anbiao, ZHANG Haizhou, XU Chongen, LI Decai, FAN Quanxin, ZOU Chengwei. Effect of pulmonary artery perfusion with HTK solution  to congenital heart disease patients with pulmonary hypertension [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(6): 88-91.
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