JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (8): 64-68.doi: 10.6040/j.issn.1671-7554.0.2016.676

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Effects of estradiol on Th1 cell polarization and liver fibrosis

ZHAN Fengxia1, DING Juan2, MA Beilei2, WANG Qian2   

  1. 1. Department of Clinical Laboratory, Campus Clinic of Shandong University, Jinan 250100, Shandong, China;
    2. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2016-06-06 Online:2016-08-10 Published:2016-08-10

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Abstract: Objective To explore the effects of estradiol(E2)on Th1 cell polarization and liver fibrosis. Methods A total of 47 patients with liver fibrosis were divided into 2 groups according to the level of E2: group A(elevated E2 level, n=19)and group B(normal E2 level, n=28). Another 30 healthy people were enrolled as the negative controls(group C). The T lymphocyte subsets were measured with flow cytometry. The levels of hyaluronic acid(HA), laminin(LN), procollagen type Ⅲ amino-terminal peptide(PⅢP)and type IV collagen(CⅣ)were detected with chemiluminescence. The levels of IFN-γ, IL-2, IL-4 and IL-10 were measured with ELISIA assay. Then the parameters of the three groups were compared. Results The E2 level was higher in group A than in group C and group B(P<0.001). The levels of HA, LN, PIIIP and CⅣ in group A were all higher than those in group C(P<0.001, P=0.001, P=0.006, P<0.001), and the levels of these four parameters in group B were also higher than those in group C(all P<0.001). The level of PⅢP was higher in group A than in group B(P=0.045). The percentage of CD3+ T cells in group A was lower than that in group C and group B respectively(P<0.001, P=0.001), and the percentage of CD4+ T cells was also lower than that in group C and group B(P<0.001, P=0.024), while the percentages of CD3+ T cells and CD4+ T cells in group B were all lower than those in group C(P=0.002, P<0.001); The percentage of CD8+ T cells in both group A and groupB was higher than that in group C(P=0.001, P=0.012). The level of IFN-γ in group A was higher than that in group C and group B(both P<0.001), and the level of IL-2 was also higher than that in group C and group B(P<0.001, P=0.003), at the same time, the levels of these two 山 东 大 学 学 报 (医 学 版)54卷8期 -展凤霞,等.雌二醇对Th1细胞极化与肝纤维化形成的影响 \=-parameters in group B were all higher than those in group C(P=0.001, P=0.012). The level of IL-4 in group A was lower than that in group C and group B(P=0.002, P=0.016), and the level of IL-10 was also lower than that in group C and group B(P<0.001, P=0.012). Conclusion E2 played a dual role in the formation of liver fibrosis. Elevated level of E2 accelerated liver fibrosis, and affected the immune function via Th1 cell polarization.

Key words: Estradiol, Th1 cell, CD4+T cell, CD8+T cell, Liver fibrosis

CLC Number: 

  • R575.2
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