JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (2): 68-74.doi: 10.6040/j.issn.1671-7554.0.2015.081

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Effects of P38 mitogen-activated protein kinase signal pathway on the expression of urokinase-type plasminogen activator in ovarian cancer

ZHANG Chunxia1, ZOU Cunhua1, SONG Dongdong2, YU Jiang1   

  1. 1. Department of Genaecology and Obstetrics;
    2. Department of General Surgery, Shengli Oil Field Central Hospital, Dongying 257000, Shandong, China
  • Received:2015-01-21 Online:2016-02-10 Published:2016-02-10

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Abstract: Objective To explore the effects of P38 mitogen-activated protein kinase(P38MAPK)signal pathway on the expression of urokinase-type plasminogen activator(uPA)in ovarian cancer. Methods The expressions of uPA, P38MAPK, extracellular regulated kinase(ERK), and AKT/kinase B in 49 cases of ovarian cancer were detected with immunohistochemistry. The expressions of uPA and P38MAPK in ovarian cancer cell line HO-8910 and HO-8910PM were detected with Western blotting. Changes of uPA were observed after P38MAPK, ERK and AKT signal pathway were blocked by SB203580, U0126 and MK-2206 respectively. Results The results of immunohistochemistry showed that positive expression rates of uPA, P38MAPK, ERK and AKT were 61.22%, 57.14%, 53.06%, and 55.10%, respectively. The expression of the uPA was related to clinicopathologic stage, differentiation and metastasis(P<0.05), and positively correlated with P38MAPK(P=0.01), but not related to ERK or AKT(P>0.05). The expressions of AKT and ERK were related to lymph node metastasis and greater omentum metastasis(P<0.05). The expression of uPA and P38MAPK in HO-8910PM was higher than those in HO-8910, and it reduced when the P38MAPK signal pathway was blocked by SB203580. But it had no significant change after dealing with U0126 and MK-2206 respectively. The postoperative survival rate was negatively correlated with P38MAPK and uPA. Conclusion The 山 东 大 学 学 报 (医 学 版)54卷2期 -张春霞,等.P38MAPK信号通路对卵巢癌中尿激酶型纤维蛋白酶原激活剂的影响 \=-P38MAPK signal pathway are activated in ovarian cancer and it can upregulate the expression of uPA, but ERK and AKT signal pathway are not involved in the regulation of uPA expression, which indicates that P38MAPK and uPA may play an important role in the invasion, metastasis and prognosis assessment of ovarian cancer.

Key words: Ovarian tumor, Urokinase-type plasminogen activator, P38MAPK signal pathway, Extracellular regulated protein kinases, Protein kinase B

CLC Number: 

  • R737.31
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