JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (7): 48-52.doi: 10.6040/j.issn.1671-7554.0.2015.184

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Occurrence of epithelia-mesenchymal transition in endometrial adenocarci-noma and the roles of miR200a/ZEB1 signaling pathway in this process

ZHANG Xiaohui, YAN Lei, QI Shasha, LU Zhenzhen, LI Mingjiang, ZHAO Xingbo   

  1. Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2015-02-13 Revised:2015-04-22 Online:2015-07-10 Published:2015-07-10

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Abstract: Objective To investigate the occurrence of epithelia-mesenchymal transition (EMT) in endometrial carcinoma and examine the roles of miR200a/zinc finger E-box-binding protein 1(ZEB1) signaling pathway in this process. Methods Different groups of samples were collected, including paraffin sections of endometrial adenocarcinoma tissues (group A, n=22), endometrial tissues after resection of uterine myoma(group B, n=45), endometrial adenocarcinoma samples obtained during operation(group C, n=15) and healthy endometrial tissues(group D, n=34). Expressions of E-cadherin, Vimentin and ZEB1 in group A and group B were investigated with immunohistochemistry. Expressions of E-cadherin, Vimentin and ZEB1 and their mRNA in group C and group D were evaluated with Western blotting and Real-time RT-PCR. Expression of miR200a in group C and D was determined with Real-time RT-PCR. Results ① Compared with group A, group B had significantly decreased expression of E-cadherin, and markedly increased expressions of Vimentin and ZEB1(both P<0.05). ② Compared with group C, group D had decreased E-cadherin and its mRNA expressions but increased expressions of Vimentin and its mRNA, and ZEB1 and its mRNA (all P<0.05). ③ Compared with group C, group D exhibited remarkably elevated miR200a, which was positively correlated to E-cadherin's mRNA(r=0.63, P<0.01)as indicated by a linear regression analysis. Conclusions EMT occurs in endometrial adenocarcinoma. The miR200/ZEB1signaling pathway can inhibit the important molecular markers in EMT.

Key words: Epithelia-mesenchymal transition, Endometrial andenocarcinoma, Zinc finger E-box-binding protein 1, microRNA200a, Regulation

CLC Number: 

  • R711.74
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