JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (10): 35-39,44.doi: 10.6040/j.issn.1671-7554.0.2014.247

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Effect of slow-released betamethasone dipropionate microparticles on neuropathic pain in rat spinal cord and expression of extracellular signal-regulated kinase

DING Chao1, HU Qiya1, HUANG Haizhen1, GUO Yaqiu1, PEI Miao1, ZHU Qingzeng2, QI Feng1   

  1. 1. Department of Anesthesiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, China
  • Received:2014-04-21 Revised:2014-09-05 Online:2014-10-10 Published:2014-10-10

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Abstract: Objective To investigate the effect of betamethasone dipropionate-ploy ethylene glycol-poly lactic acid(BDP-PEG-PLA) microparticles on the neuropathic pain in rat sciatic nerve induced by chronic constriction injury (CCI), and on the expression of extracellular signal-regulated kinase (ERK). Methods A total of 3 BDP-PEG-PLA microparticles were prepared, which were respectively denoted as the PLA number-average molecular weight 20 kD, 30 kD and 70 kD. Altogether 96 Wistar rats were randomly divided into 6 groups (n=16): sham operation group (SH group), model group (CCI group), betamethasone dipropionate group (BDP group), 20 kD group, 30 kD group, and 70 kD group. Mechanical withdraw threshold (MWT) and thermal withdrawal latency (TWL) of the rats were measured on preoperative day 1, postoperative day 3, 5, 7, 10, 13 and 15. Activation of spinal ERK was assessed on postoperative day 3 and 10 by immunohistochemistry. Results Compared with that of the CCI group, the analgesic effectof BDP group continued over a period of 5 days (P<0.05); the analgesic effects of 20 kD group, 30 kD group and 70 kD group lasted for 7, 10 and 13 days, respectively (P all <0.05). The expression of spinal pERK was suppressed in BDP group, 20 kD group, 30 kD group and 70 kD group on postoperative day 3 (P<0.05); pERK expression in 70 kD group was lower than that in CCI group and BDP group on postoperative day 10 (P<0.05). Conclusion Single injection of BDP-PEG-PLA microparticles in sciatic nerves provides long-term analgesic effect by suppressing spinal ERK activation in CCI rats, which is related to the number-average molecular weight of microparticles.

Key words: Betamethasone dipropionate, Microparticle, Chronic constriction injury, Poly ethylene glycol-poly lactic acid, Extracellular signal-regulated kinase

CLC Number: 

  • R614
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