JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Extraction of total proteins from demodex and qualification of their molecular weights

ZHU Xiaoli1, GUO Shuling1, SU Lei1, FENG Yuxin2, YUAN Fangshu1   

  1. 1. Institute of Pathogenic Biology; 2. Institute of Cell Biology, School of Medicine,
    Shandong University, Jinan 250012, Shandong, China
  • Received:2013-11-25 Online:2014-05-10 Published:2014-05-10

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Abstract: Objective  To establish the optimal extraction method of demodex protein and to analyze its components. Methods  Demodex proteins were extracted by self-made lysate and RIPA lysate, and the extraction effects were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Partial protein stripes were identified with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The NCBInr database was searched by Mascot software to analyze the components of demodex protein. Results  The extraction effect of self-made lysate was better than that of RIPA protein lysate. A total of 21 proteins were primarily identified. Conclusion  The protein components of demodex were extracted in our experiment, which is helpful for the future study on demodex proteomics.

Key words: Sodium dodecyl sulfate polyacrylamide gel electrophoresis, Molecular weight, Demodex, Proteomics

CLC Number: 

  • R384.4
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