Abstract:

Objective  To explore the protective effects of shuxuening injecta on cultured vascular endothelial cell injury due to iodine excesses.  Methods  HUVEC was cultured to 24h with iodine (4.0, 5.0, 6.0, and 8.0μmol／L) and shuxuening injecta (0, 2, 5, and 10μL/mL). Morphology and number of HUVEC, and the MTT activities and superoxide dismutase(SOD) of the culture medium on 4, 8, 12, and24 h were determined.  Results  1. Poison granules were found in the endothelium of the 5.0μmol/L groups and increased with an increase of time and dose of iodine. The above was significantly alleviated in the shuxuening groups and decreased with an increase of time and dose of shuxuening(P＜0.05). 2. With an increase of dose and over time of shuxuening, the iodine dose to inhibit the cell growth was increased(P＜0.05). 3. SOD activity positively correlated with iodine dose and was not changed in the shuxuening group(P＜0.05).   Conclusion   Shuxuening injecta can counteract injury due to iodine excesses in a dose- and time-dependent manner. Shuxuening may enhance cell anti-oxidization so as to reduce injury induced by iodine excess.

Key words: Iodine； Human umbilical vein endothelial cells； Free radicals； Proliferation； Ginkgo biloba extract