Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (6): 41-45.doi: 10.6040/j.issn.1671-7554.0.2021.1333

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Human leukocyte antigen-G promotes trophoblast invasion by regulating decidual natural killer cells

SUN Lina1,2, DU Xiaoxiao3, ZHANG Hongjuan4, MENG Jinlai2,3   

  1. 1. Shandong University of Traditional Chinese Medicine, Jinan 250013, Shandong, China;
    2. Department of Obstetrics and Gynecology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, Shandong, China;
    3. Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong, China;
    4. Department of Obstetrics and Gynecology, Tengzhou Maternal and Child Health Hospital, Tengzhou 277599, Shandong, China
  • Published:2022-06-17

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Abstract: Objective To investigate the effects of decidual natural killer(dNK)cells on the invasion ability of trophoblast cells, and to explore the mechanism of human leukocyte antigen-G(HLA-G)regulating the invasion of trophoblast cells by dNK cells. Methods The first trimester decidua tissue(n=11)was obtained from women undergoing elective termination of normal pregnancies. dNK cells were extracted with flow cytometry, and co-cultured with HTR-8/Svneo cells. The co-cultured cells were divided into HLA-G group and anti-HLA-G group, which was treated with HLA-G antibodies. Additionally, untreated HTR-8/Svneo cells were cultured as the blank control. The invasion ability of HTR-8/Svneo cells was detected with Transwell assay. The concentrations of angiopoietin-2(Ang-2)and granulocyte-macrophage colony-stimulating factor(GM-CSF)in the supernatant of co-cultured cells were determined with ELISA. Results Compared with the blank control, HLA-G group had significantly reduced invasion of HTR-8/Svneo cells(P<0.001). Compared with the HLA-G group, anti-HLA-G group had significantly reduced invasion of HTR-8/Svneo cells (P<0.01). ELISA assay results of the supernatant of co-cultured cells showed that the expressions of Ang-2(t=2.370, P=0.028)and GM-CSF(t=2.209, P=0.039)were significantly lower in the anti-HLA-G group than in the HLA-G group. Conclusion dNK cells can significantly inhibit the invasion of HTR-8/Svneo cells, and HLA-G might promote the invasion of trophoblast cells by up-regulating the expressions of Ang-2 and GM-CSF in dNK cells.

Key words: Trophoblast invasion, Human leucocyte antigen-G, Decidual natural killer cell, Preeclampsia, Angiopoietin-2, Granulocyte-macrophage colony-stimulating factor, Mother-fetal interface

CLC Number: 

  • R714.2
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