Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (12): 15-22.doi: 10.6040/j.issn.1671-7554.0.2020.1081

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Effects of Lycium barbarum seed oil(LBSO)on anti-inflammation in the aging Sertoli cells of mice(TM4 cells)

YANG Zhangjie1, WANG Yuxin2, CHEN Dongmei3, ZHAO Shuai4, HU Na4, MA Lianghong3, MA Huiming2,4   

  1. 1. School of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    2. Key Laboratory of Fertility Preservation and Maintenance(NXMU), Ministry of Education, Yinchuan 750004, Ningxia, China;
    3. General Hospital of Ningxia Medical University, Yinchuan 750000, Ningxia, China;
    4. School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, Ningxia, China
  • Published:2020-12-08

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Abstract: Objective To investigate the effects of Lycium barbarum seed oil(LBSO)on anti-inflammation in the sub-acutely aging Sertoli cells of mice(TM4 cells)induced by D-Galactose(D-Gal)and to explore the mechanism. Methods TM4 cells were treated with different concentrations(50, 100, 150 and 200 mmol/L)of D-Gal for different periods(24 h, 36 h, 48 h)in vitro. The optimal intervention time and concentration of D-Gal were determined with CCK-8 and β-galactosidase staining to establish the TM4 aging model. The expressions of aging-related factors P16INK4A, P21Waf1/Cip1, H2A.X, and β-galactosidase were detected with Western blotting. The TM4 aging models were intervened with different concentrations(1%, 3%, 5%, 10%)of LBSO containing serum in advance, and the optimal concentration was determined with CCK-8. The expressions of interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), interleukin-10(IL-10), heme oxygenase-1(HO-1)and the relative expression of p-P65/P65 were detected with Western blotting. The expression of NF-κB was located with immunofluorescence(IF). Results The inhibition rate of TM4 cells was(14.890±0.892)% when cells were treated with D-gal at 200 mmol/L for 48 h(F=12.820, P=0.0040; t=17.230, P<0.001). β-galactosidase staining indicated when compared with control group, the stained cells had increased positive rate ［(63.000±9.388)% vs(11.390±2.959)%, t=5.243, t=5.243, P<0.001)］. The expressions of P16INK4A(t=2.364, P=0.033), P21Waf1/Cip1(t=2.908, P=0.011), γH2A.X(t=2.511, P=0.025)and β-galactosidase(t=2.299, P=0.037)significantly increased in the aging model. CCK-8 detection showed a significant increasing trend in cell activity when 3% LBSO containing serum interfered with aging model ［(1.941±0.021)vs(1.784±0.019), t=5.399, P<0.001］, while the expressions of P16INK4A(t=7.211, P<0.001), P21Waf1/Cip1(t=8.248, P<0.001), γH2A.X(t=4.145, P<0.001)and β-galactosidase(t=7.288, P<0.001)downregulated. Compared to the model group, after 3% LBSO intervention, TM4 cells showed decreased expressions of TNF-α(t=4.992, P<0.001), IL-1β(t=2.921, P=0.011), IL-6(t=4.985, P<0.001), and p-P65/P65(t=24.630, P<0.001), but increased expressions of HO-1(t=6.047, P<0.001)and IL-10(t=2.315, P=0.036). Conclusion LBSO containing serum at concentration of 3% has an obvious anti-inflammatory effect on aging TM4 cells. The possible mechanism is down-regulation of NF-κB inhibits the expressions of IL-1β, TNF-α and IL-6, but activates the expressions of HO-1 and IL-10.

Key words: Lycium barbarum seed oil, TM4 cells, Inflammatory factors, NF-κB pathway

CLC Number: 

  • R277.5
[1] 陈祥宁, 刘洋, 纪俊峰. 清除衰老细胞在衰老与老龄化相关疾病中的研究进展[J]. 生物化学与生物物理进展, 2019, 46(12):1150-1161. CHEN Xiangning, LIU Yang, JI Junfeng, et al. Research progress in clearance of senescent cells in aging and age-related diseases [J]. Progress in Biochemistry and Biophysics, 2019, 46(12):1150-1161.
[2] Ruan L, Zhang X, Li R. Recent insights into the cellular and molecular determinants of aging [J]. J Cell Sci, 2018, 131(3).:jcs210831. doi: 10.1242/jcs.210831.
[3] 邓春华, 张弛, 谢云, 等. 睾丸衰老的研究现状与展望[J]. 中华男科学杂志, 2020, 26(4):291-296. DENG Chunhua, ZHANG Chi, XIE Yun, et al. Researches on testicular aging: Status quo and prospects [J]. National Journal of Andrology, 2020, 26(4): 291-296.
[4] Nozaki S, Naiki T, Naiki-Ito A, et al. Selective lysine-specific demethylase 1 inhibitor, NCL1, could cause testicular toxicity via the regulation of apoptosis [J]. Andrology, 2020. doi: 10.1111/andr.12846.
[5] Zhang M, Park H J, Seo S, et al. Evaluation of toxicity of 4-octylphenol in TM4 Sertoli cells: an in vitro study. [J]. Toxicology mechanisms and methods, 2019, 29(8): 623-631.
[6] Wu Z, Luby-Phelps K, Bugde A, et al. Capacity for stochastic self-renewal and differentiation in mammalian spermatogonial stem cells [J]. J Cell Biol, 2009, 187(4): 513-524.
[7] 王烁程, 陈晓丽, 张林波, 等. 支持细胞对精原干细胞增殖、分化调控的研究进展[J]. 畜牧兽医学报, 2019, 50(2):253-260. WANG Shuocheng, CHEN Xiaoli, ZHANG Linbo, et al. The Progress of the Study on the Regulation of the Proliferation, Differentiation of Spermatogonial [J]. Chinese Journal of Animal and Veterinary Sciences, 2019, 50(2): 253-260.
[8] Gao Y, Wei Y, Wang Y, et al. Lycium Barbarum: A Traditional Chinese Herb and A Promising Anti-Aging Agent [J]. Aging Dis, 2017, 8(6): 778-791.
[9] 杨文博, 李红兵, 吴璟, 等. 枸杞籽油对C57BL/6J小鼠2型糖尿病肾脏损伤干预作用的研究[J]. 宁夏医科大学学报, 2012, 34(1):1-3. YANG Wenbo, LI Hongbing, WU Jing, et al. Protective effect of Lycium Barbarum seed oil on renal lesion in c57bl/6J mice with type 2 diabetic [J]. Journal of Ningxia Medical University, 2012, 34(1): 1-3.
[10] 段雨劼, 胡余明, 姚松银, 等. 枸杞籽油和亚麻籽油混合物对小鼠增强免疫功能的影响[J]. 中国食品卫生杂志, 2016, 28(6):743-746. DUAN Yujie, HU Yuming, YAO Songyin, et al. Effects of mixture of lycium seed oil and flaxseed oil on immune function in mice [J]. Chinese Journal of Food Hygiene, 2016, 28(6): 743-746.
[11] Souri Z, Wierenga A, van Weeghel C, et al. Loss of BAP1 is associated with upregulation of the NFkB pathway and increased HLA class I expression in uveal melanoma [J]. Cancers(Basel), 2019, 11(8). 1102.doi: 10.3390/cancers11081102.
[12] Lu L, Wu C, Lu BJ, et al. BabaoDan cures hepatic encephalopathy by decreasing ammonia levels and alleviating inflammation in rats [J]. J Ethnopharmacol, 2020, 249: 112301. doi: 10.1016/j.jep.2019.112301.
[13] Potterat O. Goji(Lycium barbarum and L. chinense): Phytochemistry, pharmacology and safety in the perspective of traditional uses and recent popularity [J]. Planta Med, 2010, 76(1): 7-19.
[14] 郑婕, 薛旻秋, 权洪峰, 等. 枸杞籽油对CUS致小鼠应激行为的保护作用[J]. 中国药理学通报, 2019, 35(1): 110-116. ZHENG Jie, XUE Mingqiu, QUAN Hongfeng, et al. Beneficial effects of Lycium barbarum seed oil on chronically stressed mice [J]. Chinese Pharmacological Bulletin, 2019, 35(1): 110-116.
[15] Astram A, Khadijah A, Yuri P, et al. Effective dose and adverse effects of maintenance Bacillus Calmette-GueRin in intermediate and high risk non-muscle invasive bladder cancer: a meta-analysis of randomized clinical trial [J]. Acta Med Indones, 2014, 46(4): 298-307.
[16] Wang Y, Yang Z, Yang L, et al. Liuweidihuang pill alleviates inflammation of the Testis via AMPK/SIRT1/NF-kappaB pathway in aging rats [J]. Evid Based Complement Alternat Med, 2020, 2020: 2792738. doi: 10.1155/2020/2792738.
[17] Spagolla N-T-R, Maria-Engler S-S, Colepicolo P, et al. Skin irritation testing beyond tissue viability: fucoxanthin effects on inflammation, homeostasis, and metabolism [J]. Pharmaceutics, 2020, 12(2): 163. doi: 10.3390/pharmaceutics12020136.
[18] Alam MB, Chowdhury NS, Sohrab MH, et al. Cerevisterol alleviates inflammation via suppression of MAPK/NF-kappaB/AP-1 and activation of the Nrf2/HO-1 Signaling Cascade [J]. Biomolecules, 2020, 10(2): 199. doi: 10.3390/biom10020199.
[19] Uekawa N, Nishikimi A, Isobe K, et al. Involvement of IL-1 family proteins in p38 linked cellular senescence of mouse embryonic fibroblasts [J]. FEBS Lett, 2004, 575(1-3): 30-34.
[20] Jetka T, Nienaltowski K, Winarski T, et al. Information-theoretic analysis of multivariate single-cell signaling responses [J]. PLoS Comput Biol, 2019, 15(7): e1007132. doi: 10.1371/journal.pcbi.1007132.
[21] Oeckinghaus A, Ghosh S. The NF-kappaB family of transcription factors and its regulation [J]. Cold Spring Harb Perspect Biol, 2009, 1(4): a34. doi: 10.1101/cshperspect.a000034.
[22] Russo M A, Sansone L, Carnevale I, et al. One special question to start with: can HIF/NFkB be a target in inflammation? [J]. Endocr Metab Immune Disord Drug Targets, 2015, 15(3): 171-185.
[23] Salminen A, Huuskonen J, Ojala J, et al. Activation of innate immunity system during aging: NF-kB signaling is the molecular culprit of inflamm-aging [J]. Ageing Res Rev, 2008, 7(2): 83-105.
[24] Karin M, Ben-Neriah Y. Phosphorylation meets ubiquitination: the control of NF-[kappa] B activity [J]. Annu Rev Immunol, 2000, 18: 621-663. doi: 10.1146/annurev.immunol.18.1.621.
[25] Chen X, Zhang C, Wang X, et al. Juglanin inhibits IL-1beta-induced inflammation in human chondrocytes [J]. Artif Cells Nanomed Biotechnol, 2019, 47(1): 3614-3620.
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