TRB3在APP/PS1转基因小鼠脑内的表达及其意义

doi:10.6040/j.issn.1671-7554.0.2015.1062

Abstract

Abstract: Objective To explore the expression of endoplasmic reticulum stress factor TRB3 in the brain of APP/PS1 transgenic mice of different months, and to clarify its relation with the progression of Alzheimers disease. Methods APP/PS1 transgenic mice in 3 and 7 months old were used in this study and wild type(WT)C57/BL6J mice were used as control group. Mice were divided into 3-month old WT group, 3-month old APP/PS1 group, 7-month old WT group and 7-month old APP/PS1 group, and each group had 10 mice. Behavioral changes of mice were determined by Morris Water Maze, and the expression of TRB3 in the brain was studied by immunohistochemistry and Western blotting. Results Compared with WT mice of same months, there were no significant behavioral test changes in 3-month old APP/PS1 group(P>0.05), and the decline of learning and memory ability was found in 7-month old APP/PS1 group, which showed statistical difference compared with WT mice of same months(P<0.05). TRB3 was mainly expressed in brain cortex. Compared with WT mice of same months, TRB3 expression increased significantly in the brain of both 3 and 7-month old APP/PS1 mice(P<0.05), and the expression level of TRB3 in the brain of 7-month old APP/PS1 group was significantly higher than that of 3-month old APP/PS1 group(P<0.05). Conclusion TRB3 shows higher expression level in the brain of APP/PS1 transgenic mice and its increase may be related to the progression of Alzheimers disease.

Key words: Endoplasmic reticulum stress, TRB3, Alzheimers disease, APP/PS1 transgenic mice

CLC Number:

R741.02

LI Maoyu, TANG Shi, DU Yifeng. Expression and significance of TRB3 in the brain of APP/PS1 transgenic mice[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 1-5.

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