Abstract:

Objective   To prepare paclitaxel-loaded mixed micelles, which were made of D-α-tocopherol polyethylene glycol 1000 succinate (TPGS), phosphatidylcholine (PC), sodium cholate (NaC) and pluronicF127-poly (ethylenimine) (PEI), and to investigate its characteristics and cytotoxicity against cancer cells. Methods  Paclitaxel-loaded mixed micelles were prepared by film dispersion method. Zeta potential and the diameter distribution of mixed micelles were measured using laser size scattering determinator. Morphology of micelles was observed with transmission electron microscope. The release behavior of drug-loaded micelles in vitro was evaluated with dialysis method. The cytotoxicity of paclitaxel-loaded mixed micelles against MCF-7/Adr cancer cells in vitro was determined. Results  The mixed micelles were almost spherical with an average diameter of 34.8nm, and were stable in media modeling physiological conditions. The cytotoxicity against MCF-7/Adr cancer cells in vitro was remarkably higher than that of the free drug. Conclusion  F127-PEI /TPGS/NaC/PC mixed micelles are a potential drug delivery system for anticancer drugs.

Key words: Cytotoxity, F127-PEI modified, Long circulating, D-α-tocopherol polyethylene glycol 1000 succinate, Paclitaxel