JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (10): 20-24.doi: 10.6040/j.issn.1671-7554.0.2014.284

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Hsp70/CD80 DNA vaccine inhibits asthma by regulating the balance of Th1/Th2/Treg/Th17 in an acute mouse model

LI Yan1, XIE Min2, SHI Xiaoling1, WANG Xiaoyan1, TANG Li1, ZHONG Sen3, CHEN Zhuang1   

  1. 1. Molecular Medicine Experimental Center, Affiliated Hospital of Luzhou Medicine College, Luzhou 646000, Sichuan, China;
    2. WestChina-Frontier Pharmaceutical Technology Company, Chengdu 610041, Sichuan, China;
    3. Affiliated Hospital of Chengdu University of TCM, Chengdu 610075, Sichuan, China
  • Received:2014-05-04 Revised:2014-09-19 Online:2014-10-10 Published:2014-10-10

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Abstract: Objective To explore the mechanism of Th1/Th2/Treg/Th17 rebalance in an acute asthma mouse model treated with heat shock protein70 (Hsp70)/CD80 DNA vaccine. Methods Forty BALB/c mice were divided into the control group, asthma-model group, pVXA1-vector group and vaccine treatment group, 10 in each group. They were intraperitoneally injected into ovalbumin (OVA) to build acute asthma model and treated with DNA vaccine. The airway responsiveness was evaluated after inhaling the methacholine(Mch). The IgE content in serum was detected by ELISA. The pathological change of lung tissue was observed by HE and AB-PAS staining. The expressions of interferon-γ(IFN-γ), interleukin-4 (IL-4), ransforming growth factor beta (TGF-β) and interleukin-17 (IL-17) in BALF were measured by ELISA. The expressions of T-bet, GATA binding protein 3 (GATA-3), forkheah box protein3 (Foxp3) and retinoid related orphan receptor gamma t (RORγt) in lung tissue were detected by Real-time PCR. Results Compared with asthmatic mouse, mouse treated with vaccine exhibited the decreased airway responsiveness, IgE (all P<0.05) and inflammatory infiltration of lung tissues, repressed proliferation of goblet cells, and increased IFN-γ/IL-4, TGF-β/IL-17, T-bet/GATA-3 and Foxp3/RORγt (all P<0.05). Conclusion The Hsp70/CD80 DNA vaccine plays a therapeutic role in asthma by restoring the balance of Th1/Th2/Treg/Th17 cell.

Key words: CD4+CD25+ Regulatory T cell, T helper cell 17, Asthma

CLC Number: 

  • R392.9
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