JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2013, Vol. 51 ›› Issue (7): 1-5.

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Protective effects of rhEPO on oxidative stress damage in cardiomyocytes and on heart after myocardial infarction

WANG Fu1, LIU Shan-wen1, LI Bin2, GENG Hai-hua3, SUN Shuai1, LI Rui1, XIAO Jie1， JI Xiao-ping1   

  1. 1.Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, China;
    2. Department of Health, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China;
    3. Department of Cardiology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China
  • Received:2012-11-15 Online:2013-07-10 Published:2013-07-10

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Abstract:

Objective   To study the protective effects of recombinant human erythropoietin(rhEPO)on cardiomyocytes against oxidative damage and on mice hearts after myocardial infarction. Methods   Isolated neonatal rat cardiomyocytes were treated with rhEPO in vitro after the establishment of oxidative damage experimental models. Thiazolyl blue (MTT) assay and flow cytometry (FCM) were used to detect the apoptosis rate of cardiomyocytes. Protein expression levels of caspase-3, Bax and Bcl-2 were detected by Western blotting. Mice were randomly divided into the sham group, the myocardial infarction group and the rhEPO treatment group. Myocardial infarction models were induced by the ligation of the left anterior descending artery in mice. Mouse heart was harvested after 14 days. HE staining was used to investigate the effects of rhEPO on the myocardial infarct size. Results   In the process of oxidative damage, rhEPO could significantly reduce the apoptosis rate of cardiomyocytes and the expressions of caspase-3 and Bax, but could raise the expression of Bcl-2. HE staining showed that rhEPO could significantly reduce the myocardial infarct size of mouse heart. Conclusion   RhEPO can protect cardiomyocytes from oxidative stress damage induced by H2O2 and reduce the myocardial infarct size in mice after myocardial infarction.

Key words: Recombinant human erythropoietin; Cardiomyocytes; Oxidative stress damage; Myocardial infarction

CLC Number: 

  • R542.2
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