Abstract:

Objective   To elucidate the effects of GATA-4 on MSCs survival ability in hypoxic condition. Methods   Rat MSCs were transfected with GATA-4 gene, and MSCs transfected with blank plasmid were regarded as contrast.The GATA-4 transfection effect was detected by   Western blotting.The MSCs were cultured after exposure to hypoxia for 48h to imitate the myocardial infarction oxidative stress injury in vivo.The morphology of MSCs was observed. The rate of MTT cell proliferation, the positive rate of annexin V-PE apoptosis detection and the level of LDH were tested to evaluate the MSCs survival ability. Results   The positive rate of annexin V-PE apoptosis and the level of LDH significantly decreased and the rate of MTT cell proliferation increased in GATA-4 transduced MSCs(MSCGATA-4) than in the control group(MSCNull).The difference was statistically significant(P＜0.05). Conclusion   GATA-4 promotes MSCs hypoxia resistance and survival ability, which provides a new pathway to treat ischemic cardiomyopathy by MSCs transplantation and increase cell survival ability.

Key words: GATA-4; MSCs; Hypoxic condition; MSCs transplantation; Ischemic cardiomyopathy