JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2013, Vol. 51 ›› Issue (11): 42-45.

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Effect of Rabdosia lophanthoides on the proliferation and apoptosis in hepatoma carcinoma cell line HepG2

CHEN Yuan-hong1, ZENG Yi1, LUO Yan-hong2   

  1. 1. Department of Microbiology and Immunology； 2. Department of Laboratory Medical Science,
    Youjiang Medical School for Nationalities, Baise 533000,  Guangxi， China
  • Received:2013-02-04 Online:2013-11-10 Published:2013-11-10

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Abstract:

Objective  To investigate the effect of Rabdosia lophanthoides on the proliferation and apoptosis in hepatoma carcinoma cell line HepG2. Methods  The effect of Rabdosia lophanthoides on the cell proliferation and cell apoptosis was detected by the MTT method and flow cytometry.The expressions of Bax and Bcl-2 protein were detected by the ELISA method. Results  After incubation with different concentrations of Rabdosia lophanthoides, the growth of hepatoma carcinoma cell line HepG2 was significantly inhibited in a time- and dose-dependent manner(P<0.01). After the cells were treated with 0.75g/μL Rabdosia lophanthoides for 3 and 6 h, the apoptosis was induced. The apoptosis of hepatoma carcinoma cell line HepG2 was significantly inhibited in a time-dependent manner. Compared with the negative control group, the cell apoptosis was induced significantly (P<0.05). After treated with 0.75g/μL Rabdosia lophanthoides for 6h, the expression of Bcl-2 protein decreased, and the expression of Bax protein increased.The difference had statistical significance (P<0.05). Conclusion  Rabdosia lophanthoides can inhibit cell proliferation of hepatoma carcinoma cell line HepG2 in a time- and dose-dependent manner. The mechanism might relate to inducing cell apoptosis, decreasing the expression of Bcl-2 and increasing the expression of Bax.

Key words: Human hepatocellular carcinoma; Rabdosia lophanthoides; Proliferation; Apoptosis

CLC Number: 

  • R735.7
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