JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (9): 11-.

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Inhibitory effects of RNA interference and Erlotinib by blocking epidermal
growth factor receptor pathway on the proliferation of A549 cells

LIU Qing-liang1, MU Xiao-yan1, WANG-Jing2, CHI Xiang-yu2, ZHANG Min3, MA Wei-xia3   

  1. 1. Department of Health Comprehensive Ward One, Jinan 250013, China; 2. Department of Health Respiratory, Jinan
    250021, China；  3. Department of Respratory, Jinan 250021, China, Provincial Hospital Affiliated to Shandong University
  • Received:2012-02-27 Online:2012-09-10 Published:2012-09-10

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Abstract:

Objective   To explore the inhibitory effect of RNAi or Erlotinib by blocking epidermal growth factor receptor pathway on the proliferation of A549 cells. Methods   We divided the A549 cells into the control group, the RNA interference group and the Erlotinib group. The inverted microscope was used to detect the morphological changes of A549 cells before and after blocking epidermal growth factor receptor pathway. RT-PCR was used to detect the expression of EGFR mRNA. The apoptosis rate of A549 cells was measured by flow cytometry. The expressions of EGFR, Bcl-2 and Bax were detected by Western blot. Results   The expression of EGFR mRNA in the RNA interference group was down-regulated. Compared with those of the control group,the apoptosis rate of A549 cells and the expression of Bax were higher,the expression of EGFR, Bcl-2 and the ratio of Bcl-2/Bax were lower in the RNA interference group and the Erlotinib group. Conclusion    Blocking EGFR pathway can promote the apoptosis of A549 cells. The mechanism may be related to the down-regulating of the ratio of Bcl2/Bax.

Key words: Lung neoplasms;  Receptor,epidermal growth factor;  Apoptosis ；A549 cells; RNA interference; Erlotinib

CLC Number: 

  • R734.2
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