Abstract:

Objective   To build an effective controlled release system for Temozolomide(TM) stable delivering with low initial burst and less neurotoxicity. Methods   TM-loaded PPC fiber was manufactured by electrospinning. Influences of fabricating parameters on drug release behavior were  investigated. The micro-morphology of TM/PPC-fibers was researched using scanning electron microscopy(SEM). Some fiber sheets were then coated with ALG.  In vitro, the release speeds of two forms of samples were studied and their cytotoxicity against C6 glioma cells was assessed. Results   Uniform and smooth TM/PPC-fibers could be fabricated only when the weight concentration was 8%, the distance was 15-20cm and the voltage between the nozzle and the collection instrument was 10-15kV. The fiber diameter was about 3μm. The sustained release time was 12 days.The initial bursts of drug-fiber sheets were reduced after being covered with ALG. Cell toxicity test results showed that the above two forms of TM/PPC-fibers can continuously inhibited the C6 glioma cell, and the TM/PPC-fiber sheets were strongly cytotoxic. Conclusions   Electrospinning is a feasible method to fabricate TM/PPC-fibers, and ALG covering reduces the initial burst, making the TM release curve more smooth.

Key words: Temozolomide; Poly-propylene carbonate; Electrospinning; Glioma; Alginate