JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (6): 70-.

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Effects of BNIP3 on autophagy of hepatoma cells: a study using
BNIP3 over-expression vectors and BNIP3-shRNA vectors

SUN Lei， WEI Qing， ZHANG Ying， HAN Li-hui   

  1. Department of Immunology, School of Medicine, Shandong University, Jinan 250012, China
  • Received:2012-01-06 Online:2012-06-10 Published:2012-06-10

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Abstract:

Objective   To explore the effects of BNIP3 on autophagy of hepatoma cells by using over-expression vectors and shRNA vectors. Methods   BNIP3 cDNA was extracted from human hepatoma cell line BEL-7402 and was cloned into the pcDNA3.1-His-C vector to construct an over-expression vector(pcDNA3.1-BNIP3). The recombinant shRNA expression vector targeting the BNIP3 gene was also constructed(pSliencer-BNIP3). Both recombinant plasmids were verified by sequencing, enzymatic digestion, RT-PCR and Western blot. The effects of BNIP3 on autophagy of hepatoma cells were investigated by transfecting BEL-7402 hepatoma cells with the recombinant plasimids. Results   Autophagy of hepatoma cells was significantly enhanced with pcDNA3.1-BNIP3 and compromised with pSilencer-BNIP3. Conclusion   BNIP3 was involved in promoting autophagy of hepatoma cells.

Key words: BNIP3； Plasmid； shRNA； Autophagy; Carcinoma, hepatocellular

CLC Number: 

  • R392.11
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