JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (6): 31-.

• Articles • Previous Articles     Next Articles

Research advances on in vitro culture techniques of corneal epithelial cells

WANG Rui-xin, FAN Ting-jun   

  1. Laboratory of Corneal Tissue-engineering, College of Marine Life Sciences, Ocean University of China,
    Qingdao 266003, Shandong, China
  • Received:2012-03-12 Online:2012-06-10 Published:2012-06-10

PDF (PC)

502

Abstract:

In vitro reconstruction of tissue-engineered corneas for transplantation is an effective way to cure corneal blindness, and the key issue is the source of corneal epithelial seed cells. Due to the short life-span of corneal epithelial cells in vitro, improvement of culture techniques to obtain the cells with strong proliferation ability is believed to solve the seed cells source problem. In this present paper, we reviewed the in vitro culture techniques of corneal epithelial cells including culture methods and conditions.

Key words: Tissue-engineering cornea; Corneal epithelial cells; Seed cells; In vitro culture

CLC Number: 

  • R318
[1] LI Mian-xian, CHEN Tan, LI Hong-xia. Discussion of GATA-4 improving MSCs hypoxia resistance [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(7): 6-9.
[2] LIU Meng-meng1, LUAN Bao-hua1, SUN Liang1, LI Zhong-hua2, WANG Xiao-xia3. Construction of cartilage with cocultures of mesenchymal-derived  cartilage seed cells and acellular allogenic cartilage in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(7): 24-28.
[3] CHEN Shi-min1，2, XIN Jia-xuan1, XU Chun-xiao1, LIU Bin1, SUN Xiao-ming2, HU Cheng-jin2, LIU Xian-xi1. Effects of UbcH10 expression on proliferation of colorectal cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(1): 35-38.
[4] CUI Jun1, XU Xin1,2, MA Yue1, DONG Pengfei3, LIU Gangli1, WEN Yong1. A preliminary experimental study on bone marrow stromal cell biocompatibility of carboxymethyl chitosan calcium [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(6): 57-60.
[5] LI Deqiang1, LIU Peilai1, TANG Tingting2, LU Jianxi2, ZHANG Yuankai1, LI Deqiang1, LIU Peilai1, TANG Tingting2, LU Jianxi2, ZHANG Y. Effects of dynamic culture on the osteogenic differentiation of BMSCs in the 3D porous scaffold [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(4): 49-53.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd