JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (5): 60-.

• Articles • Previous Articles     Next Articles

Isolation of human glioma-derived stem cells and
establishment of an invasive glioma model

WANG Zhi-gang， JIANG Bin， MENG Qing-hu， HAO Xiao-guang， DING Xuan， LI Ji-sen   

  1. Department of Neurosurgery, The Second Hospital of Shangdong University, Jinan 250033, China
  • Received:2011-12-01 Online:2012-05-10 Published:2012-05-10

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Abstract:

Objective   To isolate brain tumor stem cells(BTSCs)from glioblastoma(GBM)specimens, and to develop an infiltrative GBM model. Methods   Glioma neural spheres were isolated from 12 glioma tissues. The cells were observed for proliferation, self-renewal and differentiation. Confirmation was done by testing for expression of  specific molecular markers. These cells’ functionality in initiating tumors was examined in immune-deficit mice. Results   Glioma neural spheres were isolated from 9 out of the 12 samples and were able to express specific molecular markers. These cells had the capacity of proliferation, self-renewal and differentiation. Further, these cells were found to be able to initiate GBM-like tumors in mice brains. Conclusion   We successfully isolated BTSCs from human glioblastoma (GBM) samples. An invasive glioma model was established.

Key words: Glioma; Stem cells; Culture and identification; Transfection; Animal model

CLC Number: 

  • R651
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