JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (5): 65-69.

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Effect of rHuEPO on the proliferation and TGF-β mRNA expressions of
rat mesangial cells induced by high glucose

WANG Lei, GUAN Guang-ju   

  1. Department of Nephrology, The Second Hospital of Shandong University, Jinan 250033, China
  • Received:2012-02-15 Online:2012-05-10 Published:2012-05-10

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Abstract:

Objective   To investigate the effect of recombinant human erythropoietin( rHuEPO )on high glucose-induced proliferation and TGF-βmRNA expressions in rat renal mesangial cell (MCs).  Methods   Cultured RMCs were stimulated by different concentrations of high glucose (10, 20, 30, 40 and 50mmol/L). 30mmol/L was the fit stimulation concentration, and then different concentrations of rHuEPO(1, 10, 50, 100 and 1000U/mL)were added into MCs of glucose induced rats. The MCs proliferative rates within 24h, 48h and 72h were detected by the Cell Counting Kit-8 (CCK-8) colorimetric assay. The TGF-βmRNA level in cells was detected by real-time PCR. Results   Within the range of 30mmol/L, glucose could stimulate the MCs proliferation. The best concentration and the best culture time were 30mmol/L and 72 hours respectively. Within the range of 100IU/ml, different concentrations of rHuEPO could inhibit the high glucose induced MCs proliferation and inhibit TGF-βmRNA expressions. Conclusions   High concentrations within 30mmol/L can stimulate MCs proliferation. Different concentrations of rHuEPO within 100IU/ml can stimulate the high glucose induced proliferation of MCs and inhibit TGF-βmRNA expressions.

Key words: Recombinant human erythropoietin; Mesangial cell; Proliferation; TGF-βmRNA

CLC Number: 

  • R587.1
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