Abstract:

Objective   To examine whether calcitonin gene-related peptide (CGRP) can induce the production of chemotactic cytokines ligand 27(CCL27) by the human keratinocyte cell line HaCaT， and to identify the mechanism of the signal pathway involved in the effect of CGRP. Methods   Expression of CCL27 mRNA in CGRP-stimulated cultural HaCaT cells was analyzed by reverse transcription PCR( RT-PCR). The concentration of CCL27 in the supernatants was measured by the ELISA method. Protein expression levels of phosphorylated p38 MAPK (p-p38 MAPK) were detected by Western blotting． Results   Incubation with CGRP increased not only mRNA expressions and protein levels of CCL27 in supernatants, but also  expression of p-p38 MAPK. Pretreatment with SB203580 and CGRP8-37 decreased CGRP-induced expression of p-p38 MAPK and the CCL27 mRNA level. SB203580 and CGRP8-37 decreased the concentration of CCL27 in supernatants. Conclusion   CGRP can induce CCL27 secretion of HaCaT cells through p38 MAPK phosphorylation.

Key words: Calcitonin gene-related peptide； Chemotactic cytokines ligand 27； p38 mitogen-activated protein kinase； HaCaT cell