JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (4): 38-41.

• Articles • Previous Articles     Next Articles

Clinical significance of HMGB1 expression in
hepatocellular carcinoma cells

WANG Xu,  CHENG Bao-quan,  LIANG Xu-yang,  JIA Jun-ying,  LI Qian-qian   

  1. Department of Gastroenterology， Qilu Hospital of Shandong University， Jinan  250012, China
  • Received:2011-10-27 Online:2012-04-10 Published:2012-04-10

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Abstract:

Objective   To investigate expression of high mobility group protein box 1 (HMGB1) in human hepatocellular carcinoma cells. Methods   PCR and Western blot were used to study HMGB1 gene expression at the mRNA and protein levels in hepatocellular carcinoma cells (LM3 and HEPG2). A normal cell line (LO2) was used as a control. Results   Expression levels of the HMGB1 gene (mRNA and protein) were much higher in hepatocellular carcinoma cells LM3 and HEPG2 than in LO2 (P<0.05). Moreover, HMGB1 expression was also higher in the highly metastatic hepatocellular carcinoma LM3 cells than in the less metastatic hepatocellular carcinoma HEPG2 cells(P<0.05).  Conclusion   High expression of HMGB1 in hepatocellular carcinoma cells suggests that it may play an important role in the growth and metastasis of hepatocellular carcinoma.

Key words: High mobility group protein box 1; Hepatocarcinoma; Metastasis

CLC Number: 

  • R735.7
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