JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (4): 33-37.

• Articles • Previous Articles     Next Articles

Effect of down-regulating SnoN gene expression on proliferation
and apoptosis of HepG2 cells

WANG Kun-hong1， WANG Wen-qi2， XIANG Yu-fen1， WANG Yi-guo2,
LIU Chang-hong2, LI Shuang-ling2, CAO Li-li3   

  1. 1. School of Medicine, Shandong University, Jinan 250012, China；
    2. Department of Gastroenterology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China；
    3. Central Laboratory, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China
  • Received:2011-09-26 Online:2012-04-10 Published:2012-04-10

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Abstract:

Objective   To explore the biological influence of small interfering RNA(siRNA) down-regulating SnoN gene expression on biological function of human HepG2 cells, such as proliferation and apoptosis. Methods   Three siRNAs targeting SnoN were designed and synthesized. The siRNAs were transiently transfected into HepG2 cells by cationic liposomes, and then the inhibition effect was detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The SnoN-siRNA with the most powerful inhibition was selected. The proliferation of HepG2 cells was examined by CCK-8, and the apoptosis was examined by flow cytometry. Results   All three siRNAs efficiently inhibited expression of the SnoN gene, and the siRNA-C had the highest inhibition ratio. The proliferation of HepG2 cells was significantly inhibited(P<0.05) and the apoptosis increased(P<0.05) after SnoN was down-regulated. Conclusion   SnoN specific siRNA can effectively inhibit expression of SnoN in human HepG2 cells, and induce growth inhibition and apoptosis.

Key words: Small interfering RNA； Genes, SnoN； Liver neoplasms； Apoptosis

CLC Number: 

  • R735.7
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