Abstract:

Objective   To investigate the levels of platelet activation indexes including soluble P-selectin (sP-selectin) and soluble CD40L (sCD40L) in subjects with different glucose metabolisms, and analyze the influencing factors for further study. Methods   The study enrolled pre-diabetic, T2DM patients and normal controls (NC) with 30 subjects in each group. Body mass index (BMI) was calculated. Fasting plasma glucose (FPG), insulin (FINS), triglyceride (TG), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C) and glycated hemoglobin (HbA1C) levels were measured. Soluble P-selectin and soluble CD40L in serum were measured by enzyme-linked immunosorbent assay. Moreover, HOMA-IR of insulin resistance was calculated by homeostasis models. Results   The sCD40L and sP-selectin levels in pre-diabetes and T2DM groups were significant higher compared with those of the NC group (P<0.05). The sP-selectin level was positively correlated with FPG, TG, TC, LDL-C and HOMA-IR (P<0.05), in addition, its correlation with TG and FPG was especially higher (P<0.01).But it was negatively correlated with HDL-C (P<0.05). Furthermore, the sCD40L level was positively correlated with FPG, BMI, and HbA1c (P<0.05). However, it was not associated with TG, TC and HOMA-IR (P>0.05). Conclusions   The sCD40L and sP-selectin levels significantly increase in pre-diabetic and diabetic subjects, which suggests platelet activation has occurred during the pre-diabetic stage.

Key words: Type 2 Diabetes, Pre-diabetes, Soluble P-selectin, Soluble CD40L, Platelet activation