JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (3): 108-112.

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Isolation of endothelial cells from hepatocellular carcinoma and
their PRL-3 expression

ZHAO Wen-bo1， LIU Xin1， LIU Xiao-qian2， CUI Xin1， WANG　Xin1   

  1. 1. Department of Hematology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China；
    2. Department of Hematology, Yantai Yuhuangding Hospital, Medical college,  Qingdao University,
    Yantai 264003， Shandong， China
  • Received:2011-07-30 Online:2012-03-10 Published:2012-03-10

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Abstract:

Objective   To investigate PRL-3 expression in endothelial cells isolated  from hepatocellular carcinoma (HCC) and its correlation  with angiogenesis. Methods   Endothelial cells were isolated from freshly collected liver tissues of 30 HCC cases using anti-CD31 conjugated magnetic microbeads. PRL-3 expression in the isolated cells was examined by real-time PCR and Western blotting. Transcript abundances of MMP-2 and MMP-9 in endothelial cells were investigated by real-time PCR. Results   The isolated endothelial cells exhibited a typical “cobblestone” appearance. Immunostaining for the von Willebrand factor proved positive in more than 90% of the isolated cells, with their PRL-3 expression being significantly up-regulated (PRL-3 vs β-actin: 11.50±2.95 vs 1.76±1.04, P<0.001). Conclusion   The results strongly suggested that endothelial cells’ PRL-3 is involved in the angiogenesis of HCC and, hence, could be a useful marker for antiangiogenesis therapy.

Key words: Hepatocellular carcinoma; Endothelium; Phosphatase of regenerating liver 3; Angiogenesis

CLC Number: 

  • R735.7
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