Abstract:

Objective   To investigate changes of serum visfatin and tumor necrosis factor-α(TNF-α)in patients with polycystic ovary syndrome(PCOS) and their significances in pathogenesis of PCOS． Methods   50 cases of PCOS patients were collected as the experimental group and 48 subjects as the control group. According to the body mass index(BMI), which was calculated by measuring height and body weight, they were subdivided into obese (BMI≥25kg/m2) and non-obese (BMI＜25kg/m2) groups, that was 23 cases in the obese experimental group，27 cases in the non-obese experimental group, 20 cases in the obese control group, and 27 cases in the non-obese control group. Fasting serum visfatin was detected by enzyme linked immunosorbent assay(ELISA), TNF-α by radioimmunoassay (RIA), fasting plasma glucose(FPG) by glucose oxidation enzyme method, and fasting insulin(FIN) by electrochemiluminescence immunoassay method. The homeostasis model assessment-insulin resistance(HOMA-IR) was analyzed. Results   Visfatin and TNF-α levels in the experimental group were higher than those in the control group(P<0.05). The level of visfatin was higher in both obese and non-obese experimental groups than in the non-obese control group, and it was higher in the obese experimental group than in the obese control group. The level of TNF-α was higher in obese and nonobese patients than in the corresponding control groups, and it was higher in the non-obese experimental group than in the obese control group (P<0.05). Visfatin and TNF-α levels were both positively correlated with BMI and HOMA-IR (P<0.05). Conclusion   Increases of visfatin and TNF-α levels in PCOS patients are closely related with HOMA-IR. Visfatin and TNF-α play important roles in occurrence and development of obesity and IR in PCOS patients.

Key words: Polycystic ovary syndrome； Visfatin； Tumor necrosis factor-α； Insulin resistance