Abstract:

Objective   To study the inhibitory effect and apoptosis-inducing action of acetidin extractive of Radix Actinidiae on transplantation tumor of lung cancer A549 cells in nude mice, and to explore their mechanisms. Methods   A model of transplantation tumor bearing lung cancer A549 cells was established in nude mice, and the effect of acetidin extractive of Radix Actinidiae in vivo was detected. The control group and treatment groups (highdose group and low-dose group)were set for comparison．According to the transplantation tumor volume, the transplantation tumor growth curve was drawn. According to the final tumor weight, the inhibitory ratio was calculated. Terminal deoxynucleotidyl transferase mediated UTP nick end labeling (TUNEL) was used to determine the apoptosis index of transplantation tumor. Expressions of Survivin protein and mRNA in transplantation tumor were detected by immunohistochemistry (SP) and RT-PCR, respectively. Results　The growth of transplantation tumor in treatment groups was slow after taking drug. The final tumor volume and tumor weight in treatment groups were smaller than those in the control group. Tumor weight inhibitory rates in the high-dose group and low-dose group were 69.00% and 45.09%, respectively. The apoptosis index of transplantation tumor in treatment groups was higher than that in the control group. Expression of Survivin mRNA and protein in treatment groups were lower than those in the control group, and they was more signifi-cantly decreased in the high-dose group. Differences were significant in the above indexes between each two groups(P<0.01). Conclusion   The acetidin extractive of Radix Actinidiae has an inhibitory effect on growth of transplantation tumor of lung cancer A549 cells in nude mice, inhibiting proliferation activity of transplantation tumor, degrading expressions of Survivin protein and mRNA, and inducing apoptosis of cancer cells.

Key words: Radix Actinidiae extractive; Lung neoplasms; Mice, nude; Apoptosis; Gene, Survivin; Immunohistochemistry; Reverse transcriptase polymerase chain reaction