JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (2): 34-37.

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Generation of reactive oxygen species in apoptosis of human gastric cancer
SGC-7901 cells induced by 5,7-Dihydroxy-8-nitrochrysin

JIANG Chong-hui1, AI Xiao-hong2, YANG Xiao-hong3, LUO Qiong3, CAO Jian-guo3   

  1. 1. Department of Pharmacy, Hengyang Central Hospital, Hengyang 421001, Hunan, China;
    2. Department of Oncology, First Affiliated Hospital of Nanhua University, Hengyang 421001, Hunan, China;
    3. School of Medicine, Hunan Normal University, Changsha 410013, China
  • Received:2011-09-20 Online:2012-02-10 Published:2012-02-10

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Abstract:

Objective   To investigate induction of apoptosis and generation of reactive oxygen species (ROS) of human gastric cancer SGC-7901 cells in vitro by 5,7-dihydrox-8-nitrochrysin(NOC). Methods   SGC-7901 cells were cultured in vitro and the percentage of Sub-G1 cell population was detected by PI stained flow cytometry (FCM). Cell histone-associated DNA fragments were examined by ELISA assay. The intracellular accumulation of ROS was determined by FCM using the fluorescent probes H2DCFDA. Results   NOC significantly increased the percentage of Sub-G1 cell population and histone-associated DNA fragments in SGC-7901 cells in a concentration-dependent manner (P＜0.01). NOC also markedly promoted accumulation of ROS(P＜0.01). The pre-incubation of N-acetylcysteine, an antioxidant, effectively antagonized the induction of apoptosis of SGC-7901 cells by NOC. Conclusion   NOC induced apoptosis of SGC-7901 cell line is associated with induction of ROS generation.

Key words: Gastric neoplasms; Chrysin; Chrysin derivatives; Apoptosis; Reactive oxygen species

CLC Number: 

  • R735.2
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