Abstract:

Objective   To introduce a recombinant VP1 gene of EV71 into the Bacillus subtilis genome by means of homologous recombination to display VP1 on the spore as a mucosa vaccine for enterovirus 71. Methods   A recombinant VP1 gene fused with the Bacillus subtilis CotB gene was introduced into integration vector pDG1662 to  generate plasmid pDG1662-CotB-VP1. Bacillus subtilis 1A771 was transformed with linearized pDG1662-CotB-VP1 by means of electroporation. Recombinant strains were screened by antibiotic resistance and were identified by PCR, Western-Blot and immunofluorescence microscopy. Result   The VP1 gene was integrated into the Bacillus subtilis genome and the VP1 protein was displayed on the spore′s surface. Conclusion   The success  of VP1 protein expression on the spore′s surface is a major achievement toward constructing a mucosa vaccine for enterovirus 71.

Key words: Enterovirus 71； Virus structure protein VP1；  Mucosa vaccine； Bacillus subtilis spores