JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (12): 47-51.

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Model construction of transplanting rat embryonic skin
precursor complex in nude mice

LI Chuan, CHENG Yan-na, JIANG Du-yin   

  1. Department of Burn and Plastic Surgery, Second Hospital of Shandong University, Jinan 250033, China
  • Received:2012-04-16 Online:2012-12-10 Published:2012-12-10

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Abstract:

Objective   To screen the optimal pregnancy time window of transplanting rat embryonic skin precursor tissue in nude mice, and explore a new method for studying and intervening embryonic skin and appendage development. Methods   Rat embryonic skin precursor complexes(ESPCs) of different gestational ages(E14.5d, E16.5d and E18.5d) were acquired with little subcutaneous tissue, then they were implanted under dorsal flap of nude mice. 7 days after transplantation, the flap was opened and living ESPCs was exposed. The histological characteristics of fetal skin and development of appendages were observed. Results   E16.5d and E18.5d grafts survived successfully and continued developing, and finally generated intact skin structure with skin appendages. E14.5d grafts generated into teratoid neoplasm. Conclusion   With the methods of implanting under flap, ESPCs can successfully survive, and E16.5d is the optimal time for transplanting ESPCs.

Key words: Embryos; Skin appendages; Development; Animal model

CLC Number: 

  • R644
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