JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (1): 62-65.

• Articles • Previous Articles     Next Articles

Regulatory role of the let-7/miR-98 family in the IL-6 expression

WANG Shu-jun， GENG Qin， ZHANG Xiu-ying, ZHANG Hong-ju, WEI Qiao-feng, CUI Yan-hui   

  1. Department of Rheumatology, Central Hospital of Zibo, Zibo 255000, Shandong, China
  • Received:2011-06-17 Online:2012-01-10 Published:2012-01-10

PDF (PC)

217

Abstract:

Objective   To investigate the regulatory role of microRNA in expression of interleukin-6(IL-6). Methods    The let-7/miR-98 family was predicted to regulate IL-6 by bioinformatics. Real-time PCR and ELISA were used to determine IL-6 mRNA and protein expression levels, respectively. Site-directed gene mutagenesis and reporter gene assay were performed to identify the direct regulatory effect of let-7/miR-98 on IL-6 3′UTR. Results   miR-98 can inhibit the IL-6 expression at both protein and mRNA levels. Furthermore, let-7/miR98 can directly target IL-6 3′UTR. Conclusion   Let-7/miR-98 down-regulates the IL-6 expression. The findings indicate that let-7/miR-98 may provide a novel strategy for therapeutic intervention of IL-6--realated diseases.

Key words:  microRNA; let-7/miR-98 family; Genes, interleukin-6

CLC Number: 

  • R392.11
[1] FAN Ming-de1, ZHANG Yuan2, MIAO Bao-wang1, ZHENG Kun-peng1, LI Ke1, WANG Cheng-wei1. Expression of Nod-like receptor pyrin domain-containing protein 3 (NLRP3) in glioma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(4): 51-54.
[2] LIU Xiang-lan, JIANG Yang, WANG Qun, ZHU Fa-liang, DONG Zhao-jing, GUO Chun, ZHANG Li-ning. The influence of PDCD4 gene to the differentiation of
T lymphocytes subsets [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(11): 6-.
[3] SUN Lei， WEI Qing， ZHANG Ying， HAN Li-hui. Effects of BNIP3 on autophagy of hepatoma cells: a study using
BNIP3 over-expression vectors and BNIP3-shRNA vectors [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(6): 70-.
[4] JIANG Ya-li1, TAO Yan-ting1, HU Xiao-yan1, XU Chun-xiao2, ZHANG Lian-ying1. Construction of the recombinant plasmid pEGFP-N1-ZIP2 and
its effect on expression of other zinc transporters in
 human T lymphocyte and monocyte cell lines [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(4): 24-28.
[5] CUI Bin1，2, WANG Laicheng2, ZHU Min2, JIAO Yulian2, XIN Wei2,MA Chunyan2, ZHANG Jie2, ZHAO Yueran2. Construction of recombinant prokaryotic expression plasmid of human high mobility group box1 and its expression and purification [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(4): 40-44.
[6] WANG Jin-Jin, SHI Wei, QI Jian-Ni, LUAN Fang, ZHAO De, GUO Chun, MA Chun-hong. Cloning of the human ZHX2 functional domain [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(5): 1-4.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd