JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (6): 84-88.

• Articles • Previous Articles     Next Articles

Effects of trichostatin A and docetaxel on apoptosis and  α-tubulin in lung adenocarcinoma A549 cells

ZHANG Qun-cheng, MA Han-chen, SHAO Yang, ZHANG Dong, JIANG Shu-juan   

  1. Respiratory Department, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China
  • Received:2011-03-20 Online:2011-06-10 Published:2011-06-10

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Abstract:

 Objective    To investigate the effect of administration of trichostatin A(TSA) and docetaxel(Doc) on apoptosis and α-tubulin in lung adenocarcinoma A549 cells.     Methods    The cell inhibition ratio was evaluated using MTT assay. Apoptosis was assessed using Hoechst 33258 staining and the cell cycle was detected by flow cytometry. Protein expression of Acetyl-α-tubulin was detected by immunofluorescence and Western blot. Results     Doc significantly inhibited the growth of A549 cells, and combined treatment with TSA induced severer apoptosis. The cell cycle was more markedly arrested in the G2/M phase  compared with the single drug(P＜0.05). TSA and Doc increased protein expression of Acetyl-α-tubulin. Conclusion      TSA induces sensitivity to Doc treatment in A549 cells through up-regulation of acetyl-α-tubulin.

Key words: Trichostatin A;  Docetaxel;  Tubulin

CLC Number: 

  • R734.2
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