Abstract:

Objective     To investigate effect and mechanism of Qindan capsule(QC)-containing serum on proliferation and migration of adventitial fibroblasts (AF). Methods     AFs from the thoracic aorta of rats were cultured in vitro by a tissue explant. The proliferation and migration of AF affected by siRNA-Smad2 and QC-containing serum with high ［750mg/(kg·d)］ or low doses ［150mg/(kg·d)］ were detected by MTT and Transwell. mRNA and protein expressions of Smad2 and Smad7 were observed by realtime PCR and Western blot, respectively. Results     After stimulation by TGF-β1 for 24 h, the proliferation and migration of AF were higher compared with those untreated. Knockdown of Smad2 and QC-containing serum with high and low doses inhibited proliferation and migration of AF(P<0.05). And drug-containing serum could downregulate expression of Smad2, while up-regulating expression of Smad7(P<0.01). Conclusion    QC-containing serum has a significant inhibitory effect on proliferation and migration of AF induced by TGF-β1. The mechanism may be related to the TGF-β1/ Smads signaling pathway.

Key words:  Qindan capsulecontaining serum; Smads; Adventitial fibroblasts; Proliferation; Migration