Egb761抑制谷氨酸受体拮抗大鼠海马神经元兴奋毒损伤

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (6): 15-19.

• Articles • Previous Articles Next Articles

Neur-oprotection of Egb761 against glutamate excitotoxicity through inhibiting glutamate receptors in cultured rat hippocampal neurons

LIU Yong1, GU Ying-Hui1, PENG Yan1, ZHOU Guo-ping2, ZHOU Deng-ming2, MIAO Bei3, HOU Xiao-yu1

1. Research Center for Biochemistry & Molecular Biology, Jiangsu Key Laboratory of Brain Disease Bioinformation;
2. Department of Clinical Medicine; 3. Jiangsu Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221002, Jiangsu, China

Received:2010-11-29 Online:2011-06-10 Published:2011-06-10

Abstract

Abstract:

Objective To study the mechanism of neuro-protection of ginkgo biloba leaf extract Egb761 against glutamate excito-toxicity. MethodsEffects of Egb761 on glutamate-induced apoptosis-like neuronal cell death were investigated by DAPI staining and TUNEL. Effects of Egb761 on glutamate-induced whole-cell currents were detected by patch-clamp recording in cultured hippocampal neurons. Results Egb761 could inhibit glutamate-induced apoptosis-like cell death in cultured rat hippocampal neurons. Egb761 could also dose-dependently inhibit glutamate-induced whole-cell currents, including NMDA receptors and kainate acid(KA) receptors. Conclusion Egb761 could protect cultured hippocampal neurons against glutamate excitotoxicity through blocking iontrophic glutamate receptors.

Key words: Egb761; Receptors, N-methyl-D-aspartate; Receptors, kainate acid; Excitotoxicity; Patch-clamp techniques

CLC Number:

Q593+.2

LIU Yong1, GU Ying-Hui1, PENG Yan1, ZHOU Guo-ping2, ZHOU Deng-ming2, MIAO Bei3, HOU Xiao-yu1. Neur-oprotection of Egb761 against glutamate excitotoxicity through inhibiting glutamate receptors in cultured rat hippocampal neurons[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(6): 15-19.