JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (4): 70-74.

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Effect of SKI-II on SGC7901 cell cycle by inhibiting the Sphk1 expression

ZHU Zu-an1, FEI Su-juan1, LIU Lei2, ZHANG Qiu-yue1, SUN Min1, LIU Ying2   

  1. 1. Department of Gastroenterology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221000, Jiangsu, China;
    2. Department of Pathology, Xuzhou Medical College, Xuzhou 221000, Jiangsu, China
  • Received:2010-09-29 Online:2011-04-10 Published:2011-04-10

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Abstract:

Objective    To study the effect of SKI-II on arresting gastric cancer SGC7901 cell cycle, and explore the mechanisms. Methods    SGC7901 cells were treated with SKIII alone or in combination with cisplatin,  and the cell cycle was analyzed by flow cytometry.  Expressions of Sphk1 and P27 were detected by immunocytochemistry and Western-blot. Results     After being treated with SKI-II alone or in combination with cisplatin for 48h,  the ratio of cells arresting in the G0/G1 phase was increased, compared with the control group(P<0.05); the effect of combined treatment group was more significant(P<0.05). Expression of P27 in SGC7901 cells was increased, while expression of Sphk1 was decreased, compared with the control group(P<0.05). Pearson correlation analysis showed that expressions of Sphk1 and P27 were negatively related. Conclusion    SKI-II could arrest gastric cancer SGC7901 cell cycle and inhibit cell growth through increased expression of P27, which was regulated by inhibiting expression of Sphk1.

Key words: SKI-II; SGC7901 cells; Stomach  neoplasm； Cell cycle

CLC Number: 

  • R735.2
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