JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (4): 33-37.

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Protective effects of ginsenoside Rb1 and Re on  SK-N-SH cells injured by Aβ25-35

JIA Li-yun1, PAN Xiao-hua2, LIU Jing1, CUI Xing3, WANG Mo-lin1   

  1. 1. Institute of Medical Genetics, School of Medicine, Shandong University, Jinan 250012, China;
    2. Department of General Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;
    3. Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012,  China
  • Received:2011-01-24 Online:2011-04-10 Published:2011-04-10

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Abstract:

Objective    To investigate protective effects of ginsenoside Rb1 and Re against injury of SK-N-SH cells induced by Aβ25-35 and the possible mechanism. Methods        Aβ25-35 was added to the medium for cell culture to make a cell model of Alzheimer disease, and ginsenoside Rb1 or Re were used to treat the injured cells. Cell survival rates were determined by the MTT assay, intracellular ROS levels were determined by fluorescence probes, and expressions of phosphorylated tau and active glycogen synthase kinase 3β(GSK-3β) were determined by Western blot. Results     The survival ratio of SK-N-SH cells were significantly decreased after exposure to Aβ25-35, and treatment with ginsenoside Rb1 or Re significantly increased the survival ratio and decreased the cellular ROS level. Ginsenoside Rb1 and Re decreased expressions of phosphorylated tau and active GSK-3β, respectively. Conclusion     Ginsenoside Rb1 and Re may exert a neuroprotective effect on SHNSH neural cells induced by neurotoxic Aβ25-35.

Key words: Panax; Alzheimer disease; Tau proteins; Ca(2+)calmodulin dependent protein kinase

CLC Number: 

  • R741
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