Abstract:

Objective    To observe effects of G-CSF and VEGF on synaptic plasticity in the APP transgenic mouse model. Methods    54 10-month-old APP transgenic mice were randomly divided into 3 groups：the G-CSF combined with VEGF group, the G-CSF group and the control group. Injections of VEGF(8μg/kg·d) for 3d were peritoneally given to mice in the G-CSF combined with VEGF group, then injections of G-CSF(50μg/kg·d) for 7d were subcutaneously given; injections of PBS for 3d were peritoneally given to mice in the G-CSF group, then injections of G-CSF(50μg/kg·d) for 7d were subcutaneously given; injections of PBS for 3d were peritoneally given to mice in the control group, then injections of normal saline for 7d were subcutaneously given. Expression of CD133 was observed 7, 14 and 28 days after administration by immunohistochemistry. BrdU and synaptophysin (SYN) double expressions were detected by fluorescent staining, and influences of G-CSF combined with VEGF on proliferation of mouse stem cells and synaptic plasticity were observed. Results    Expressions of CD133 and SYN increased in the G-CSF combined with VEGF group(P<0.05).  Conclusion    G-CSF combined with VEGF may increase synaptic function and improve synaptic plasticity by promoting neurogenesis.

Key words: Granulocyte colony-stimulating factor;  Vascular endothelial growth factor; APP transgenic mouse model;  Synaptophysin