JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (1): 35-38.

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Effects of UbcH10 expression on proliferation of colorectal cancer cells

CHEN Shi-min1，2, XIN Jia-xuan1, XU Chun-xiao1, LIU Bin1, SUN Xiao-ming2,  HU Cheng-jin2, LIU Xian-xi1   

  1. 1. Institute of Biochemistry and Molecular Biology, School of Medicine,  Shandong University, Jinan 250012, China;
    2. Department of Laboratory Diagnosis, General Hospital of Jinan Military Region, Jinan 250031, China
  • Received:2009-12-26 Online:2011-01-10 Published:2011-01-10

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Abstract:

Objective    To investigate the effect of the UbcH10(Ubiquitin-conjugating enzymes) expression on the proliferation of  colorectal cancer cells. Methods    The pcDNA3.1UbcH10 and pcDNA3.1 were transfected into LoVo cells. UbcH10 expression was determined by Western blot. Proliferation of LoVo cells was detected by CCK-8 kit. The effect of UbcH10 on the cell cycle of colorectal cancer cells was assessed by flow cytometry. Results    The UbcH10 expression in LoVo cells transfected with pcDNA3.1UbcH10 was more obviously increased than that in control cells. Over-expression of UbcH10 obviously promoted cell proliferation compared with the controls.   UbcH10 over-expression significantly decreased cell cycle distribution in the G2-M phase (P<0.05). Conclusion     UbcH10 can promote proliferation of colorectal cancer cells. The potential mechanism may depend on its regulation of the cell cycle.

Key words: Ubiquitin-conjugating enzymes; Cell proliferation; Cell cycle

CLC Number: 

  • R318
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