Abstract:

Objective  To explore the inflammatory response of microglia induced by β-amyloid (Aβ) oligomers through the receptor for advanced glycation end product (RAGE) ,and to further analyze the relationship between Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway and tumor necrosis factor-α (TNF-α) of microglia induced by Aβoligomers. Methods   After the primary rat microglial cells were stimulated and obstructed, the level of TNF-α extracted from supernatant liquid of microglia was measured by enzyme-linked immunosorbent assay (ELISA). Results   TNF-αwas increased after primary rat microglia cells  were treated by Aβoligomers for 24h. However, anti-RAGE IgG and AG490 reduced the activation of microglia cells and obviously inhibited Aβ oligomer-induced release of TNF-α. Conclusions    RAGE is the receptor of microglia in inflammatory response induced by Aβ oligomer. JAK/STAT pathway may participate in the release of TNF-α induced by Aβ oligomer.

Key words: Amyloid beta-protein; Microglia; Glycosylation end products, advanced; Signal transduction; Tumor necrosis factor