Abstract:

Objective  To investigate the synergistic effects of rosiglitazone (ROZ) and cisplatin on the growth of human endometrial cancer cell line KLE xenografted to nude mice. Methods  KLE cells were transplanted into nude mice to form study model. When the models were established, they were randomly divided into 6 groups: control group(A), cisplatin1mg/kg(B), cisplatin 3mg/kg(C), ROZ 50mg/kg(D), cisplatin1mg/kg＋ROZ 50mg/kg(E), and cisplatin 3mg/kg＋ROZ 50mg/kg(F). Cisplatin was given by intraperitoneal injection every two days; ROZ was intragastric administration every day. During the period of observation, the volumes of the transplanted tumors were measured every 4 days. The tumor growth inhibitory rates of each group were calculated. Moreover, the curves of BALB/cnu mice growth were drawn. After 38 days, BALB/c-nu mice were euthanized and xenograft tumors were measured. The expression of NF-κB and PTEN protein in subcutaneous tumors were determined by immunohistochemical method with image analysis system. Results  Significant differences in tumor inhibitory rates were found between the treatment groups and the control group(P<0.05).The tumor inhibitory rate of B，C，D，E，F 5 group was 24.41%，43.34%，49.67%，78.02%，84.78%, respectively, and the combination group showed synergistic effect on inhibiting the growth of the xenografts. Contrary to control group, the expression of subcutaneous tumor NF-κB was down-regulated and PTEN was obviously up-regulated by rosiglitazone and its combination with cisplatin. Conclusion  Rosiglitazone could enhance inhibition of the growth of endometrial tumor by combination with cisplatin.

Key words: Endometrial neoplasms; Rosiglitazone; Cisplatin; Mice, nude