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Table of Content

      
    16 May 2010
    Volume 48 Issue 5
    Articles
    Levels of serum pancreatic derived factor(PANDER)and their correlations with islet β cell function in newly diagnosed type 2 diabetic patients
    ZHUANG Feng1, GUAN Qingbo1, GAO Ling1, YU Chunxiao1, TIAN Limin1, WANG Jing2, FAN Youfei1, DU Dongdong3, ZHAO Jiajun1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  1-4. 
    Abstract ( 1008 )   PDF (350KB) ( 416 )   Save
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    Objective  To determinate the levels of serum pancreatic derived factor (PANDER) in newly diagnosed type 2 diabetic patients and to investigate the relationship between islet β cell function, insulin resistance and serum PANDER.  Methods  Oral glucose tolerance and insulin releasing test was performed in 36 newly diagnosed type 2 diabetic patients(T2DM group) and 23 healthy people(NC group). Differences of parameter for blood glucose, insulin, islet β cell function and insulin resistance were compared between the two groups,and the correlation between islet β cell function, insulin resistance and PANDER was analyzed.  Results  Serum PANDER levels in T2DM group were higher than those in NC group(P<0.01);serum PANDER was independently positively correlated with islet β cell function(P<0.05),but was not correlated with insulin resistance(P>0.05).  Conclusion  PANDER may be a novel serum inflammation marker, and take part in the pathogenesis of T2DM.

    Effect of metformin on hepatic and renal function in elderly patients with type 2 diabetes mellitus
    WANG Xia, LI Li, SUN Ruixia, GAO Yanyan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  5. 
    Abstract ( 1182 )   PDF (381KB) ( 449 )   Save
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    Objective  Evaluating the safety of metformin in the treatment of elderly patients with Type 2 diabetes mellitus(T2DM). Methods  96 cases of elderly T2DM with poorly controlled blood glucose poorly controlled were randomly divided into the metformin group and the control group. The fasting blood glucose(FBG), glycosylated hemoglobin(HbA1c), lipid, hepatic and renal function and serum uric acid(UA) were determined before and after treatment, and the side effect of metformin was also observed. Result  After one year, 94 cases accomplished the observation. After treatment with metfoumin the serum creatinine, uric acid and glomerular filtration rate(GFR) had no significant changes after treatment with metformin(P>0.05); the blood urea nitrogen(BUN) significantly increased(P<0.05); the alanine aminotransferase (ALT) significantly declined (P<0.05). The GFR at different levels had no significant changes and no acidosis occurred in all patients.  Level of BUN in the metformin group was higher than that in the controls after treatment(P<0.05). Levels of hepatic function, Cr, GFR and UA had no significant difference between the two groups after treatment(P>0.05). Conclusion  If the patients have no severe hepatic and renal dysfunction, metformin is safe and effective in the treatment of elderly patients with T2DM. Metformin does not promote the reduction of hepatic and renal function. Aging is not the contraindication of metformin, but observation is needed to last for much more time.

    Effect of α-MSH on fatty acid oxidation in mouse skeletal muscle
    JIN Yongjun1,YANG Meizi2,ZHANG Lingyun1,WANG Xiuyun1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  9-13. 
    Abstract ( 1141 )   PDF (481KB) ( 422 )   Save
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    Objective  To investigate the effect and mechanism of α-Melanocyte stimulating hormone(α-MSH)on fatty acid oxidation in skeletal muscle. Methods  Saline or α-MSH were subcutaneously injected  into 8-week C57BL/6J male mice , and the carnitine palmitoyl transferase-1(CPT-1) activities were measured 0, 0.5, 1, 3 and 5 hours after treatment. Melanocortin receptor(MCR) subtypes in the muscle and C2C12 cells were measured by RT-PCR, and the effect of α-MSH on palmitate oxidation was also analyzed. Results  CPT-1 activities were significantly increased 1 and 3 hours after treatment(P<0.05). MC1R, MC3R, MC4R and MC5R were expressed in C2C12 cells and primary cultured skeletal muscle, and α-MSH increased the palmitate oxidation in a dose-dependently way. Conclusion  α-MSH might  increased fatty acid oxidation in skeletal muscle through MCR.

    Effects of diet-control, aerobic training and rosiglitazone on cardiac interstitial remodeling in high fatfed obese rats
    LI Qian, SUN Shuzhen, WANG Yi, LI Yan, CHEN Yao, NIU Na
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  14. 
    Abstract ( 1002 )   PDF (432KB) ( 378 )   Save
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    Objective  To study the intervention effects of diet-control, aerobic training and rosiglitazone on cardiac interstitial remodeling in high fat-fed obese rats. Methods  SD rats were randomly divided into the normal control group and the high fat-fed group, which were fed with a basic and high fat diet respectively for 8 weeks. High fat-fed obese rats were selected according to their body weight and then divided into obese control group, diet-control group, diet-control plus swimming group and diet-control plus rosiglitazone group. Cardiac structure, serum lipids levels, serum PAI-1 levels, cardiac collagen contents and PAI-1 mRNA expressions were measured at the 16th week. Results  Cardiac collagen contents and PAI-1 levels in serum and hearts were increased in high fat-fed obese rats with hypertrophic left ventricle(P<0.05,P<0.01). Cardiac structure, collagen accumulation and PAI-1 mRNA expressions were improved in diet-control group(P<0.01). Left ventricular hypertrophy was aggravated and PAI-1 expressions were decreased markedly in diet-control plus swimming group(P<0.01). Cardiac collagen contents and PAI-1 levels in serum and hearts were decreased significantly in diet-control plus rosiglitazone group(P<0.05,P<0.01) with remarkably rehabilitated cardiac structure. Conclusion  PAI-1 might be related to cardiac interstitial remodeling in high fat-fed obese rats. Diet-control, aerobic training and rosiglitazone therapy could improve cardiac remodeling in various degrees.

    Marchantin M displays growth Inhibitory effects on human prostse cancer cells
    XU Aihui1, PAN Zhe2, JIANG Hanming1, KONG Feng1, HU Zhimin3, YUAN Huiqing1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  18-22. 
    Abstract ( 1198 )   PDF (1277KB) ( 225 )   Save
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    Objective  To Investigate the antiproliferational effect of Marchantin M on human carcinoma cells. MethodsThe cytotoxic effect of Marchantin M was assessed by MTT assay. It′s inhibitory effect on the growth of PC-3 cells was measured by incorporation of BrdU. The cell cycle progression and apoptosis of PC-3 cells were determined by the flow cytometry assay. Alterations in the expression of cell cycle related proteins in PC-3 cells exposed to Marchantin M were determined by Western blotting. Nucleic morphological changes of Marchantin M-treated PC-3 cells were determined by staining cells with DAPI. Results  Marchantin M led to a viability inhibition of K562, HepG2, MCF-7, LNCaP, DU145 and PC-3 cells in a dose-dependent manner. However, Marchantin M had no significant inhibitory effects on the growth of normal human retina pigment epithelial cells(hTERT-RPE1). Flow cytometry assay indicated that treatment with Marchantin M resulted in cell cycle arrest at G0/G1 phase. Further examination showed that the expression of cell cycle related proteins were changed after treatment. DAPI staining assay showed that Marchantin M treatment caused the increase in fraction of apoptotic cells. Conclusion  These observations indicate that Marchantin M is a promising compound that shows antitumor activity against protate cancer cells.

    Study of combination of allicin and amphotericin B inhibitinghyphae formation of Candida albicans in vitro
    WANG Xiaoyan, QI Mei, LI Yutang, SUN Yundong, ZHANG Xiaoli, LIU Juan, CHENG Yizhe, YU Han, ZHOU Yabin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  23-27. 
    Abstract ( 1308 )   PDF (682KB) ( 305 )   Save
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    Objective  To explore combination of allicin and amphotericin B inhibiting effect of filament formation against Candida albicans in vitro, and detect cytotoxicity variation under combination of two drugs. Methods  The effect of combination of two drugs against Candida albicans was determined by the checkerboard microdilution method, and the fractional inhibitory concentration index(FICI)was calculated. The filament formation was induced in 10% calf serum-1640.The impact of combination of allicin and amphotericin B on the rate of hyphae and filament formation of Candida albicans was determined.The H8 cytotoxicity variation under combination of two drugs was detected through cellular appearance and MTT. Results  Drugs combined together acted synergistically against Candida albicans, and allicin could enhance the ability of amphotericin B to inhibit filament formation.The effect of cytotoxicity was reduced remarkably under combination of two drugs. Conclusion  Allicin largely enhances the anti-Candida albicans activity of amphotericin B, and it is a best selection in clinical applications for reducing dose and adverse effect of amphotericin B.

    Preparation of antimMIF monoclonal antibodies and its radio-iodinated labeling
    SONG Jing, ZHANG Chao, LIANG Ting, CHEN Fuqiang, HOU Guihua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  28-31. 
    Abstract ( 1164 )   PDF (602KB) ( 360 )   Save
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    Objectives  To explore the potential values of macrophage migration inhibitory factor(MIF) in inflammation and tumor radioimaging. Methods  Recombinant mMIF was obtained through IPTG-induced pEQ30-mMIF/M15 E.coli, and anti-mMIF McAb was made with hybridoma technology whose  specificity and biological activities were identified with Western blotting, ELISA and MMI. Anti-mMIF antibodies were radioiodinated by Na125I with Iodogen method. Results  Three stable cell lines were obtained, named as 5G2D7, 5G2C7 and 5G2E3 respectively with high specificity and biological activity to MIF. The labeling rate of 125I-anti mMIF McAb was 90.40%, the radiochemical purity was 98.36%, and specific radioactivity was 21.48MBq/μg.  Conclusion  Anti-mMIF McAb was obtained and radioiodinated successfully,which may supply a new tool for further research of MIF,especially for clinical oncology and inflammation radioimaging.

    Construction and identification of the human PDCD4 promotor luciferase reporter plasmid
    LIU Fengming, ZHANG Xia, SONG Xingguo, JIANG Yang, ZHANG Lining
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  32-35. 
    Abstract ( 1126 )   PDF (519KB) ( 269 )   Save
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    Objective  To construct the human programmed cell death 4 promotor luciferase reporter gene vector and detect its activity in cells. Methods  The PDCD4 promotor from human genomic DNA was amplified by PCR, and was inserted into the luciferase report gene pGL4-basic vector. The amplified DNA sequence was confirmed by sequencing. To detect the transcriptional activity of human PDCD4-P1 in the plasmid,transient transfection was performed in different cell lines, and pRL-TK was used to determine the transfection efficiency. Results  The sequencing results indicated that the amplified sequence was correct. The results of transient transfection showed that the recombinant plasmid could be highly expressed in the OVCAR3 cell line which could highly express endogenous PDCD4, but lowly in the SKOV3 cell line in which lowly expressed endogenous PDCD4 could be detected. Conclusion  The human PDCD4 promotor luciferase reporter gene vector has been constructed successfully, which will lay experimental foundation for further study.

    Inhibition of migration in ovarian cancer cell SKOV3    by gene-silence of kinesin KIF4A
    GAO Jie1, WANG Dongqiu2, TIAN Mingzhong2, ZHU Changjun2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  36-39. 
    Abstract ( 1076 )   PDF (1003KB) ( 249 )   Save
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    Objective  To study the effect  of silencing expression of kinesin KIF4A on migration of ovarian cancer cell SKOV3. Methods  Construct shRNA expression vector targeting KIF4A, and then transfect the plasmid into human ovarian cancer cell SKOV3. Generate the cell lines that stably expressed KIF4A shRNA and confirm KIF4A gene-silence in cells by Western Blotting assay. Employ the Transwell assay to evaluate the impact on migration in SKOV3 when silencing KIF4A. Results  KIF4A shRNA stably expressed cell lines were generated and Transwell assay showed that the migrated cells with expressing KIF4A shRNA we threefold faster than the Negative Control cells. Conclusion  Silencing KIF4A in human ovarian cancer cell SKOV3 increases its migration capability.

    Analyzing intracellular localization of structural domains in kinesin motor protein Kif18A
    WANG Dongqiu1, GAO Jie1, TIAN Mingzhong1, ZHU Changjun2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  40-43. 
    Abstract ( 1411 )   PDF (852KB) ( 314 )   Save
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    Objectiv  eTo explore the intracellular localization of structural domains in kinesin motor protein Kif18A in eukaryotic cells. Methods  Construct the GFP-expressing plasmids of Kif18A′s wild type and each domain including motor, stalk, tail and motor with part of stalk domain. Then transfect these plasmids into breast cancer cell MCF7 respectively. The GFP-fused proteins were detected by SDS-PAGE and Western blot. Use immunofluorescence staining technique to stain microtubules and DNA in cells. The intracellular localization of GFP-fused proteins was determined under fluorescent microscopy. Results  In interphase cells, GFP-motor domain distributes along microtubules. GFP-tail domain localizes in the nucleus as well as along microtubules and GFP-stalk domain scatters throughout the cytoplasm. In telophase cells, GFP-motor domain still distributes along microtubules. In contrast, GFP-motor with part of stalk domain concentrates on the midbody. Conclusions  The motor and tail domain of Kif18A can both associate with microtubules and the tail domain contained nuclear localization signal (NLS). Only with part of stalk region, motor domain of Kif18A is able to localize on the midbody.

    Effect of the short hairpin RNA of fragile histidine triad on proliferation and apoptosis of the gastric cancer cell line BGC-823
    LUO Qiong, XU Hongwei, HAO Jinghua, LIU Hui, ZHU Qiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  44-48. 
    Abstract ( 923 )   PDF (605KB) ( 332 )   Save
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    Objective  To construct two eukaryotic plasmids expressing short hairpin RNA (shRNA) of fragile histidine triad (FHIT) and to study their effects on proliferation and apoptosis of the gastric cancer cell line BGC-823. Methods  Specific shRNA plasmids to FHIT were constructed, and then transfected into the BGC-823 cells with lipofectamine methods. Cells were divided into four groups:the control group,the PGPU6/GFP/Neo-shNC transfected group as the negative group and the PGPU6/GFP/Neo-shRNA transfected groups .After selection with G418, the stable cell clones were attained. Expression of FHIT mRNA was determined by quantitive real-time PCR. The effect of FHIT on the growth characteristics of gastric cancer cells was observed by methyl thiazolyl tetrazolium (MTT) and flow cytomery (FCM). Results  Stable clones with shRNA-FHIT plasmids were obtained. Compared with the negative control cells, expression of FHIT mRNA was down-regulated in the shRNA-FHIT plasmid transfected cells(P<0.05). Proliferation was promoted, whereas the cell apoptosis rate was decreased. Cells at the G0/G1 cell stage decreased, while the cells at S and G2 cell stages increased. All these differences between shRNA-FHIT transfected cells and the two control groups of gastric cancer cells had statistical significances (P<0.05). Conclusions  shRNA-FHIT plasmids were successfully transfected into BGC-823 cells, and the cells which express FHIT in a stable lower level were obtained. FHIT-tageted shRNA could obviously decrease the FHIT expression in BGC-823 cells at a stable lower level, promote the proliferation of BGC-823 cells, suppress their apoptosis and weaken the G0/G1 phase block of cell cycle.

    Effects of carbon dioxide on the biological behavior of ovarian carcinoma xenografts and xenografts sensitivity to cisplatin
    WANG Jing1, ZHANG Airong1, ZHANG Jian2, LIU Jianmeng1, YIN Ting1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  49-53. 
    Abstract ( 1066 )   PDF (584KB) ( 246 )   Save
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    Objective  To investigate the effects of carbon dioxide (CO2) pneumoperitoneum environment on the biological behavior and chemotherapy drug sensitivity of human ovarian carcinoma cell (SKOV3) xenografts in nude mice. Method  sSKOV3 cells were exposed to simulated laparoscopic CO2 pneumoperitoneum environment at 12 mmHg CO2 pressure for 1h(a group)or 3h(b group). Cells in the control group (c group) were cultured in a standard CO2 incubator. The paired nude mice were randomly divided into the A, B, and C  groups (n=10 pairs). Cell suspensions were inoculated into the left scapular subcutaeous of nude mice in each group. After the tumors had formed apparently three days later, nude mice(10 pairs) were randomly divided into two groups again.A1, B1, and C1(DDP group)were intraperitoneally injected cisplatin(5mg/kg, 0.5mL, qw×3); A2, B2, and C2(nontreatment groups)were intraperitoneally injected saline(0.5mL, qw×3). All nude mice were sacrificed by dislocation on the following day of drug withdrawal. Then tumor volume and weight were measured.  Expressions of adhesion molecule (CD44v6) and nonmetastasis gene(nm23-H1)in xenograft tumors were analyzed by immunohistochemical staining. Results  ①The proliferation rate of xenograft tumors in the CO2 treated groups was higher than that in the control group (P<0.01), and that treated for 3 hours was higher than for 1 hour (P<0.01). ②Tumor sensitivity to DDP in the CO2 treated groups decreased compared with the control group (P<0.01).  ③There was no significant difference between the groups in expression of CD44v6 and nm23H1 (P>0.05). Conclusion  Carbon dioxide promotes the proliferation of human ovarian carcinoma cell (SKOV3) xenografts in BALB/c nude mice, decreases tumor sensitivity to DDP and has no effects on tumor metastasis. 

    Effect of mifepristone on proliferation and expression of CEACAM6 of human ovarian cancer cell line SKOV3
    SUN Ying, ZHENG Xiaojing, LI Mingjiang, ZHAO Xingbo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  54-57. 
    Abstract ( 995 )   PDF (559KB) ( 193 )   Save
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    Objective  To evaluate the effect of mifepristone on proliferation and expression of CEACAM6 of human ovarian cancer cell line SKOV3 in vitro. Methods  Human ovarian cancer cell line SKOV3 was cultured with different concentrations (control group, 1×10-7, 1×10-6, 1×10-5, 5×10-5mol/L) of mifepristone(MIF) in vitro; MTT was used to examine the cell growth; and the semi-quantity of CEACAM6 mRNA and protein were detected by RT-PCR and western blot. Results  Proliferation of SKOV3 cultured with 1×10-7, 1×10-6 mol/L of MIF was not significantly lower than the control group(P>0.05);5×10-5mol/L of MIF was able to significantly suppress the proliferation of SKOV3(P<0.01); Expressions of CEACAM6 mRNA and protein of SKOV3 in the control group were higher than that of 5×10-5mol/L of the mifepristone group(P<0.05). Conclusion  Mifepristone could significantly suppress the proliferation of human ovarian cancer cell line SKOV3 in vitro. The action mechanisms may be associated with the suppression of expression of CEACAM6.

    Effect of  letrozole  on  expressions  of VEGF and  MVD in endometriotic rats
    ZHANG Xuyan1, L Xiuping1, LI Mengmeng2, LIU Hua1, LI Jianchao3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  58-61. 
    Abstract ( 1083 )   PDF (480KB) ( 339 )   Save
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    Objective  To study the different expression patterns of vascular endothelial growth factor (VEGF)and microvessel density (MVD) in tissues of endometriosis in rat models after letrozole therapy, and to explore the underlying mechanism of letrozole treatment for endometriosis(EM). Methods  Rat models were established by autologous  transplantation of uterine tissues to an ectopic site beside the uterines. Then the successful rat models were randomly divided into the NS control group and the Letrozole (p.o.) treated groups.  To compare the difference of ectopic lesion volumes after the treatment,  an immunohistochemistry  method was used to detect VEGF and MVD expressions in tissues of endometriosis in the rat models.  Results  The volume of  endometriotic tissues in the letrozole groups decreased compared with the control group (P<0.05).  The inhibitory effect of the drug significantly increased with the duration of treatment. Expressions of VEGF and MVD in tissues of endometriosis decreased after using letrozole. Compared with the control group, the differences were significant (P<0.05). Conclusions  The volume of the ectopic focus in rat models  obviously was changed after letrozole treatment, and a significant effect appeared in a short time. Letrozole can treat EM through down-regulating expressions of VEGF and MVD in ectopic tissues.

    Inhibitory effect of rosiglitazone combined with cisplatin on the growth of endometrial carcinoma in Nude Mice
    ZU Zengyan1, ZHANG Guiyu1, MA Benhong1, WANG Hongxia2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  62-66. 
    Abstract ( 1032 )   PDF (1728KB) ( 394 )   Save
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    Objective  To investigate the synergistic effects of rosiglitazone (ROZ) and cisplatin on the growth of human endometrial cancer cell line KLE xenografted to nude mice. Methods  KLE cells were transplanted into nude mice to form study model. When the models were established, they were randomly divided into 6 groups: control group(A), cisplatin1mg/kg(B), cisplatin 3mg/kg(C), ROZ 50mg/kg(D), cisplatin1mg/kg+ROZ 50mg/kg(E), and cisplatin 3mg/kg+ROZ 50mg/kg(F). Cisplatin was given by intraperitoneal injection every two days; ROZ was intragastric administration every day. During the period of observation, the volumes of the transplanted tumors were measured every 4 days. The tumor growth inhibitory rates of each group were calculated. Moreover, the curves of BALB/cnu mice growth were drawn. After 38 days, BALB/c-nu mice were euthanized and xenograft tumors were measured. The expression of NF-κB and PTEN protein in subcutaneous tumors were determined by immunohistochemical method with image analysis system. Results  Significant differences in tumor inhibitory rates were found between the treatment groups and the control group(P<0.05).The tumor inhibitory rate of B,C,D,E,F 5 group was 24.41%,43.34%,49.67%,78.02%,84.78%, respectively, and the combination group showed synergistic effect on inhibiting the growth of the xenografts. Contrary to control group, the expression of subcutaneous tumor NF-κB was down-regulated and PTEN was obviously up-regulated by rosiglitazone and its combination with cisplatin. Conclusion  Rosiglitazone could enhance inhibition of the growth of endometrial tumor by combination with cisplatin.

    Experimental study on melatonin inhibiting hydrogen peroxide-induced cataract in rats
    GUO Yuanyuan,CAI Keli,JIANG Xintong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  67-71. 
    Abstract ( 1026 )   PDF (556KB) ( 463 )   Save
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    Objective  To investigate the effects of melatonin(MLT) in preventing hydrogen peroxide-induced cataract in rats. Methods  The lenses of the cultured normal Wistar rats in vitro were randomly divided into 3 groups: the control group (group A ), the group treated by hydrogen peroxide(group B), and the group treated by hydrogen peroxide and melatonin(10, 30, 50, 100 and 200μmol/L) (group C1, group C2,  group C3, group C4 and group C5). At the end of the study period (24th hour), the lenticular opacification of lenses was examined and the mean densities of black bands under the lenses were analyzed by Image-Pro Plus(IPP)6.0 software .At the same time, the levels of protein, glutathione(GSH), malondialdehyde(MDA), total antioxidiation ability(TAOC), superoxide dismutase(SOD) and catalase (CAT) were tested. Results  The lenses were observed with slit-lamp microscope after 24 hours of incubation, and there were differences in lenticular opacification between each group. The mean density of lenses in Group C was significantly higher than lenses in Group B (P<0.05). To compare with the biochemical parameters of group B, the levels of GSH, T-AOC, SOD and CAT of group C increased statistically; the level of MDA reduced statistically(P<0.05). Conclusion  Melatonin can effectively enhance antioxidant ability of the oxidative damaged lense, and also can inhibit the development of hydrogen peroxideinduced cataract of rats in vitro.

    Therapeutic effects of replenishing yin, clearing heat and promoting  blood circulating compound prescription on periodontitis in rats with diabetes and influence of IL-1β in peripheral blood
    LI Xue1, LIN Zhiyong1, ZHAO Fen1, ZHAO Min1, JI Mei1, YU Guina2, MU Wenli3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  72-74. 
    Abstract ( 972 )   PDF (1045KB) ( 195 )   Save
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    Objective  To observe the effect of Replenishing yin, Clearing heat and Promoting blood circulating Compound prescription on the rats with diabetic periodontitis and to explore its mechanism. Methods  10-week-old healthy male Wistar rats were randomly divided into the blank control group (group A), the diabetic periodontitis group (group B) and the traditional Chinese medicine treatment group (group C). After diabetic periodontitis models were established in group B and C , group C was fed with equivalent dosage of Honeysuckle, North Adenophora and Chuanxiong compound decoction, and group A and B were fed with an equal volume of normal saline. 2 months later, under anesthesia, boxes of bilateral maxillary including experimental zone molar were intercepted. HE staining was used to detect the general condtions and pathological changs by counting the number of infiltrating inflammatory cells in the gingival connective tissue and epithelial tissue and measuring the distance from mesial alveolar crest to the cemento-enamel junction of the experimental tooth. The venous blood was drawn out from the main vein of abdomen. After preparing serum, the level of IL-1β was measured by ELISA and the data were statistically analysed by SPSS10.0 software package.Results  The number ofinfiltrating inflammatory cells in the gingival tissue and the distance from alveolar crest to the cemento-enamel junction, were increased in groups B and C compared with those in group A, and there were significant differences among the three groups(P<0.05) . Level of IL1β in peripheral blood of group B was higher than those of groups A and C, and there was a significant difference(P<0.05). Conclusion  Replenishing yin, Clearing heat and Promoting blood circulating Compound prescription has curative effect on diabetic periodontitis.

    The recent ultrasound contrast of VX2 liver tumor in rabbit model after the cryosurgical treatment
    ZHANG Fengqin1, LI Li1, LI Man1, KANG Yongjun2,QIN Ling1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  75. 
    Abstract ( 1178 )   PDF (1150KB) ( 387 )   Save
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    Objective  To evaluate the role of enhanced ultrasonography in assessing the short term therapeutic response to Ar-He cryoablation for VX2 liver tumor. Methods  30 rabbits with VX2 tumor (group A) and 10 healthy rabbits (group B) underwent Ar-He cryoablation for the tumor and liver respectively. The contrast agent SonoVue and contrast pluse sequencing(CPS)software technology were used to evaluate the role of Ar-He before cryoablation and 1day, 1week and 2 weeks after cryoablation. Results   ①76.7 percent (23/30) of lesions in group A and 100.0 percent(10/10) of lesions in group B showed no enhancement within entire procedural periods, which indicated complete necrosis. ②23.3 percent (7/30) of lesions in group A showed local enhancement in the arterial phase, and new lesions found in the liver suggested tumor residue. ③The ring of inflammatory hyperemia enhanced in the arterial phase, and the enhancement signal in the portal phase and delayed phase was the same as the liver parenchyma. The imaging findings of the tumor residue were the same as non-cryoablation. Conclusion   Imaging characteristics of contrast enhanced ultrasound(CEUS) after treatment are helpful to evaluate the effect of the cryoablation for liver cancer.

    Association between rheumatoid arthritis and tumor necrosis factor receptor Ⅱ-196 site polymorphism: the Meta analysis
    GE Yongpeng, YANG Qingrui, ZHANG Yuanchao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  79-84. 
    Abstract ( 1213 )   PDF (351KB) ( 349 )   Save
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    Objective  To investigate the association between the tumor necrosis factor receptor Ⅱposition 196 (TNFRⅡ196) gene polymorphism and patients with rheumatoid arthritis (RA).  Methods    We performed a metaanalysis of the published literatures on the TNFRⅡ position 196 gene polymorphism and RA. Results  A total of nine literatures involving 2140 cases and 1297 controls were included. Comprehensive analysis showed that there was no association between TNFRⅡ196 alleles, TNFRⅡ196TG, GG genotype and RA, (OR=1.11; 95% CI=0.91-1.34; P=0.32 for TNFRⅡ196 alleles;OR=1.38; 95% CI=0.97-1.98; P=0.07 for TNFRⅡ196TG genotype;and OR=1.09; 95% CI=0.93-1.27; P=0.31 for TNFRⅡ196GG genotype. However, an association between TNFRⅡ196 alleles and TNFRⅡ196GG genotype and familial RA was found (OR=1.43; 95% CI= 1.11-1.86; P=0.006 for TNFRⅡ196 alleles and OR=2.68; 95% CI=1.39-5.17; P=0.003 TNFRⅡ196GG genotype, respectively), but not between TNFRⅡ196TG genotype and familial RA (OR=1.00; 95% CI=0.71-1.39; P=0.98). Finally we found no association between the TNFRⅡ196 alleles, TNFRⅡ196GG, TG genotype and Sporadic RA (OR=1.13; 95% CI=0.891.44; P=0.32 for TNFRⅡ196 alleles; OR=1.44; 95% CI= 0.75-2.76; P=0.27 for TNFRⅡ196GG genotype; and OR=1.03; 95% CI=0.76-1.39; P=0.86 for TNFRⅡ196TG genotype). Conclusions  This Meta-analysis suggested that there was no association between the TNFR Ⅱposition 196 gene polymorphism and RA, but the TNFRⅡ196 alleles and the GG genotype might be associated with familial not sporadic RA.

    Pyrosequencing of nucleoside (acid) analogues-resistance mutation of the P gene of hepatitis B virus
    SUN Haiping, WANG Lei, YANG Fang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  85. 
    Abstract ( 1291 )   PDF (357KB) ( 379 )   Save
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    Objective  To analyze the nucleoside(acid) analogues-resistant mutations at 10 sites of the P gene of hepatitis B virus(HBV). Methods  The 10 sites(rtI169T, rtV173L, rtL180M, rtA181V/T, rtT184G, rtA194T, rtS202I, rtM204V/I, rtN236T and M205V)of the HBV P gene were detected by Pyrosequencing in chronic hepatitis B (CHB) patients who had virologic breakthrough or inadequate virologic response during taking nucleoside(acid) analogues. Results  Lamivudine (LAM)  resistance of 8 modes at 4 sites was detected in 48 cases, in which rtM204 was the commonest. Adefovir dipivoxil ADV)resistance of 4 modes at 2 sites was detected in 10 cases, in which rtA181 was the commonest. Telbivudine (LdT)resistance of 2 modes at 2 sites was detected in 4 cases, and rtM204I existed in all of them. Entacavir (ETV) - resistance of 4 modes at 7 sites was detected in 6 cases, in which rtT184 was the commonest. Conclusions  Mutations in the HBV P gene can be detected during the nucleoside(acid) analogues treatment in CHB patients. We suggest that resistant mutations in CHB patients who developed virologic breakthrough be detected and individual therapy be performed.

    Evaluation of transcatheter closure in 90 cases ofcongenital ventricular septal defect
    ZHAO Lijian, HAN Bo, ZHANG Jianjun, ZHUANG Jianxin, JIN Youpeng, WANG Yulin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  89-92. 
    Abstract ( 1070 )   PDF (381KB) ( 414 )   Save
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    Objective  To evaluate the therapeutic effect of transcatheter closure of ventricular septal defect(VSD). Methods  During 2005 to 2007, a total of 90 cases diagnosed as congenital VSD were admitted to our department, of  which 86 cases underwent transcatheter closure succesessfully. The clinical data including electrocardiogram and transthoratic echocardiography before and after intervention, angiogram during intervention, implanted occluder devices and followup data of all patients were analysed. Results  Among the 90 patients, 40 were male and 50 were female, aged from 2  to 34 years  (mean age, 8.9 years). 87 had perimembranous ventricular septal defect, 3  intercristal ventricular septal defect, 1 residual fistula after surgery repair and 1 patent ductus arteriosus. 86 cases (95.6%) were occluded successfully. The VSD diameter was 1.58.8mm. The implanted occluder device diameter was 4-18mm. Amplatzer membranous VSD occluder were used in 17 cases,  home-made thin-waist-big-side occluder in 13 cases, home-made concentric occluder in 52 cases and home-made eccentric occluder in 2 cases. The average follow-up time was 18 months. 30 cases presented various complications including 3 cases of CRBBB, 2 case of ICRBBB, 6 cases of accelerated junctional tachycardia, which were all transient, disappearing after 1 week-3months. Residual shunt occured in 3 cases,  and 1 case had mechanical hemolysis, which resolved 7 days later after conservative therapy. Aortic valve regur-gitation occurred in 2 cases and tricuspid regurgitation occurred in 3 cases. All the regurgitation was mild to moderate and no further treatment were performed. Conclusions  Transcatheter closure of perimembraneous VSD is safe and efficacious, and the successful rate is high with rare severe complications. Conduction blockades are the main complications. It is important to be diagnosed and treated in time. Further followup studies are required to document the longterm effect on the intracardiac condution.

    Long-term prognosis of ocular myasthenia gravis in children and its related factors
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  93-96. 
    Abstract ( 1732 )   PDF (356KB) ( 498 )   Save
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    Objective  To explore long-term prognosis of ocular myasthenia gravis(OMG) in children and its related factors. Method  s88 patients aged between 2 and 14 years at onset were selected for this follow-up survey, and they were primarily diagnosed as having OMG. The follow-up time was 5 years to 10 years. At the end of the follow-up, the long-term prognosis of OMG was evaluated and its related factors were analyzed by the Logistic regression. Results  At the end of the follow-up,13 patients (14.77%) were asymptomatic after taking drugs for about two years;59 patients (67.05%) remained having OMG throughout the follow-up duration, including 26 recrudescent patients after drug withdrawal;16 patients (18.18%) converted into generalized myasthenia gravis, most of whom (9/16, 56.25%) occurred within 1 year after onset. The long-term prognosis of OMG had a significant correlation with acetylcholine receptor (AchR) antibody and thymopathy, including thymoma and thymic hyperplasia(P<0.05). Conclusion  Prognosis of OMG in children has some distinctive characteristics and the probability of OMG converting into generalized myasthenia gravis is lower than that in adults. Several factors, such as AchR antibody and thymopathy, may have an impact on the risk of OMG developing into generalized disease in children.

    Exprlssions of CD49d and CD11b on CD56brightCD16- NK cell of early pregnancy
    HUANG Xianliang1, DONG Baihua1, HU Weixu2, WANG Qingjie2, SONG Bingfeng2, WANG Chune2,LIU Jia2, KONG Beihua1, QU Xun2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  97-100. 
    Abstract ( 1502 )   PDF (959KB) ( 476 )   Save
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    Objective  To investigate the expressions of integrinα4(CD49d)and integrinαm (CD11b) on CD56brightCD16- NK cell of the peripheral blood and decidua in early pregnant women and the peripheral blood in missed abortion women. Methods  Peripheral blood samples were collected from early pregnant and missed abortion female, and uNK cells were isolated from the fresh decidua of early pregnant females by mechanical trituration and Ficoll gradient centrifugation. The fluorescent intensity of the integrines was detected by FCM. Results  Expressions of CD49d and CD11b on CD56brightCD16- NK cell of peripheral blood in early pregnant women those higher than were in the first trimester decidua( P<0.01), and also higher than those in missed abortion women (P<0.05). Conclusion  CD49d and CD11b may play an important role in the recruitment of uNK cells and participates in the maintenance of normal pregnancy.

    Therapy effect of auto-CIK on regulatory T cells in ovarian neoplasms patients
    YU Yuan1, LI Yan1, RONG Fengnian2, LIANG Jing1, LIU Xiaolin1, WANG Fuli1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  101-104. 
    Abstract ( 1284 )   PDF (575KB) ( 291 )   Save
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    Objective  To evaluate the variation of CD4+CD25+ regulatory T cells and T cell subsets affected by cytokine-induced killer cells in ovarian neoplasms patients. Methods  Thirty ovarian neoplasms patients were collected with pathologically diagnosed. The auto-CIK cells were transfused back into patients after peripheral blood was cultured and induced into CIK cells. The levels of CD4+CD25+ regulatory T cells, T cell subsets and NK cells were detected with flow cytometry two weeks later. Results  CD4+CD25+ regulatory T cells were decreased after autologous CIK cells transfusion, (P<0.01); the CD8+ cell subsets were decreased (P<0.05). Cell subsets CD3+ and CD4+, ratio of CD4+/CD8+ and NK cells were significantly increased at the same time. Conclusion  Autologous CIK cells can degrade the quantity of regulatory T cells, improve the unbalanced T cell subsets in patients with ovarian neoplasms and upgrade the quality of patients′ life to some extent.

    Expression of interleukin-17 in placenta and peripheralblood of patients with preeclampsia
    TIAN Meirong1, MA Yuyan1, WANG Leiyi2, FAN YueLian1, LIU Zhenping2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  105. 
    Abstract ( 1132 )   PDF (352KB) ( 270 )   Save
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    Objective  To investigate the relationship of IL-17 and Th17 cells with preeclampsia. Methods  Thirty-two patients with severe preeclampsia and thirty normal pregnant women as controls were investigated. ① Expression of IL-17 mRNA in placental specimens was detected by  real-time PCR; ② Concentrations of  IL-17, IL-6 and TNFα in serum were measured by ELISA. Results  Compared with the control group,  expression of IL-17 mRNA was higher in preeclampsia placentas (P<0.05);  levels of IL-17,IL-6 and TNF-α in maternal serum were higher in the preeclampsia group than in the controls (P<0.01, P<0.05, P<0.01 respectively ); the preeclampsia group, there was a positive correlation between IL-17 and IL-6(r=0.402, P<0.05). Conclusions  IL-17 and Th17 cells may contribute to the pathogenesis and pathophysiology of preeclampsia. IL-6 and TNF-α may also be involved in the process.

    The relationship of CXCR4 and MMP-9 expressions and clinico-pathologicalfactors with early recurrence after radical resection of colorectalcancer and their clinico-pathological significance
    SUN Yanlai1, LI Zengjun1, ZHENG Yan2, XU Zhongfa1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  108-112. 
    Abstract ( 1035 )   PDF (473KB) ( 294 )   Save
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    Objective  To investigate the relationship of CXCR4 and MMP-9 expressions and clinico-pathologic factors with early recurrence after radical resection of colorectal cancer. Methods  Expressions of CXCR4 and MMP-9 were detected in 40 cases of early recurrence colorectal cancer (recurrence time: postoperative 3 months to 1 year) and 30 cases of non-recurrence colorectal cancer (without recurrence for more than three years) in the same period with the SP immunohistochemical method. The χ2 test and Logistic regression analysis were used to analyze the relationship of CXCR4 and MMP-9 expressions and clinico-pathologic factors with the early recurrence of colorectal cancer. Results  The CXCR4-positive rates in the early recurrence group and the non-recurrence group were 75.0%(30/40) and (43.3%)(13/30) respectively, and there was a significant difference between the two groups(P<0.05). The MMP-9-positive rates in the two groups were 65.0%(26/40) and 40.0%(12/30) respectively, and there was a significant difference between them(P<0.05). The risk factors for postoperative early recurrence include the tumor location (P=0.004,β=1.957,OR=7.079), invasion depth of the bowel wall(P=0.047,β=0.889, OR=2.434)and CXCR4 expression (P=0.008, β=1.855, OR=6.391). CXCR4 expression and the tumor site were the most important factors for postoperative early recurrence. Postoperative adjuvant chemotherapy (P=0.026,β=-2.440, OR=0.087) was the protective factor for early recurrence. Conclusion  The abnormal expression of CXCR4 is closely associated with the early recurrence after radical resection of colorectal cancer, which indicates that CXCR4 expression may be involved in the development of colorectal cancer.

    Prostate volume, intravesical prostatic protrusion and bladder-urethra angle of  prostate as predictors of acute urinary retention in benign prostate hyperplasia
    ZHAN Lilong, XIA Qinghua, JIN Xunbo, XIONG Hui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  113-115. 
    Abstract ( 1183 )   PDF (568KB) ( 242 )   Save
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    Objective  To evaluate the value of prostate volume(PV), intravesical prostatic protrusion(IPP) and bladder-urethra angle(BUA) as predictors of acute urinary retention (AUR) in men with benign  prostate hyperhlasia(BPH). Methods  40 BPH patients were divided into 2 groups. 22 patients in AUR group had surffered AUR at least one time, while the other18 patients had never underwent AUR, measured PV、IPP 、 BUA by MRI, and make a relative analysis. Results  In the clinical data, there was no significance in the PV and IPP between two groups(P>0.05). There was a statistical significance in the BUA between two groups (P<0.05).When BUA is over 45°, there is a risk of 88.9%  developing AUR. Conclusions  BUA is one of the indicators  to predict whether BPH patients have the probability of developing AUR. BUA measured by MRI is a simple way to assess AUR in BPH patients.

    Evaluation of non specific high uptake of aged mediastinal lymph nodes by 18F-FDDG PET/CT
    LING Chunxiang, HUANG Zhaoqin, RUI Jun, LIU Qingwei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  116-120. 
    Abstract ( 1735 )   PDF (580KB) ( 341 )   Save
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    Objective  To investigate the difference of PET/CT between non-specific high uptake of the aged mediastinal lymph nodes and mediastinal lymph nodes metastases caused by malignant tumors especially lung cancer. Methods  110 patients with detailed clinical data were retrospectively studied, including 55 with non-specific high uptake of the aged mediastinal lymph nodes (group1), 53 with mediastinal lymph nodes metastases(group2), and 2 with both non-specific high uptake of the aged mediastinal lymph nodes and mediastinal lymph nodes metastases. The  SUVmax, SUVmean, length diameter, short diameter, mean CT value and maximum CT value of the lymph nodes were measured with a  blind method. Results  Obvious differences between  group 1 and the group 2 were found in SUVmax(3.98±1.48 vs 7.41±3.69), SUVmean(3.37±1.16 vs 5.88±2.64), length diameter[(10.32±3.01)mm vs (16.0±5.97mm)],short diameter[(7.48±2.29)mm vs (11.95±4.78)mm], mean CT value[(64.16±21.73)Hu vs (33.84±8.80)Hu] and maximum CT value [(111.02±149.41)Hu vs (67.93±32.45Hu)] (P<0.01). Conclusions  The non-specific high uptake of the aged mediastinal lymph nodes  usually accompanied with symmetrical distribution, SUV value lower than 5 and CT value higher than 50Hu. The SUV value and CT value (particularly the increased CT value) could be used as references in the differential diagnosis of benign and malignant lymph nodes caused by malignant  tumors especially lung cancer.

    Relationship between the degree of right coronary artery stenosis and average blood flow and pulsafility index of saphenous vein
    YANG Lieming1, MA Liming2, WU Guanghua2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  121-123. 
    Abstract ( 1214 )   PDF (330KB) ( 269 )   Save
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    Objective  To study the relationship between the degree of stenosis of right coronary stenosis and the blood flow of saphenous vein (SV) in on-pump coronary artery bypass grafting (CCABG).  Methods  According to the RCA stenosis degree, the 89 patients of CCABG treatment were divided into 3 groups (<75%, 75%95%, >95%): 15 cases in group A, 53 cases in group B, 21 cases in group C. After routine SV and RCA anastomosis is completed and then hemodynamic values were stable, the SV bridge flow pattern, mean flow, pulsatility index (PI) and mean arterial blood pressure were measured and recorded directly by transittime flowmeter to contrastively study the SV blood flow. Results  SV mean blood flow values of group C(43.8±19.1) mL/min were significantly higher than the values of group A(26.3±12.8) mL/min and group B  (35.6±17.0)mL/min(P<0.01); and SV mean blood flow values of group B was higher than the  values of group A(P<0.01). Pulsatility index (PI) of group C(1.72±0.81) was significantly lower than that of group A(3.8±1.4) and group B(2.9±0.98)(P<0.01), group B was lower than that of group A(P<0.05). But there were no statistically significant differences of three groups mean blood pressure (P>0.05). Conclusion  In on-pump coronary artery bypass grafting, the RCA stenosis degree is higher,the SV bridge blood flow is greater, and the PI values is smaller.

    Association between PSG parameters and 24 hour ambulatory blood pressure rhythm in OSAHS patients
    LIU Yunqiu, JI Li, ZHAO Ying
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  124-127. 
    Abstract ( 1052 )   PDF (328KB) ( 267 )   Save
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    Objective  To investigate the correlation between PSG parameters and high blood pressure in night and day for obstructive sleep apnea-hypopnea syndrome (OSAHS) patients. Method   223 cases were divided into three groups: the simple OSAHS group of 96 cases, the OSAHS  with hypertension of 66 cases and  the normal control group of 61 cases. All subjects were subjected to ABPM monitoring for two periods, from 7:00 to 21:00 and from 21:00 to 7:00  the next day. While these subjects were subjected to PSG monitoring from 21:00 to 7:00 on the next day . Regression analysis was separately performed to find the correlation between blood pressure (ABPM) parameters  and PSG parameters in OSAHS patients with hypertension. Result    PSG parameters of AHI, night-awakening index, below 90 percent by oxygen and oxygen saturation index were different between  the simple OSAHS group, the OSAHShypertension group and the normal control group (P<0.01). The multiple regression analysis on the correlation of DABPS and PSG parameters for OSAHS patients with hypertension showed that only nightawakening number (times/H) was introduced into the regression equation(partial regression coefficient=0.55249, P=0.0001); of DABPS, only the aerobic index was introduced into the regression equation(partial regression coefficient=0.34201, P=0.0293); of NABPS, microawakening index, apnea+low ventilation and oxygen by the time were introduced into the regression equation(partial regression coefficient=0.34216, P=0.0287;  partial regression coefficient=0.46876, P=0.0308; partial regression coefficien=0.40204, P=0.0249); of DABPS, apnea hypopnea+total time, 90 percent oxygen by saturation index (time/h) and oxygen index were introduced into the regression equation(partial regression coefficien=0.39443, P=0.0390;partial regression coefficient=0.39142, P=0.0399; partial regression coefficient=0.40360, P=0.0246).  Conclusions  The blood pressure of OSAHS patients lost normal rhythm variation, representing that the blood pressure increases in day and night.For all OSAHS patients, the higher the AHI, night-awakening index, 90 percent low saturation index and oxygen desaturation index, the more probably the patient sufferes from hypertension.For OSAHS patients with hypertension, daytime shrink pressure positively correlated with night-awakening index; daytime diastolic pressure is positively correlated with oxygen desaturation index; night shrink pressure is positively correlated with night-awakening index, apnea+hypopnea total time and oxygen desaturation index; the night diastolic pressure is positively correlated with apnea+hypopnea total time, 90 percent low saturation index and oxygen desaturation index.

    MRI features of extra-abdominal desmoid-type fibromatoses
    YANG Hui, YANG Chenming, YU Dexin, LI Chuanfu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  128. 
    Abstract ( 1247 )   PDF (958KB) ( 332 )   Save
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    Objective  To investigate the magnetic resonance imaging (MRI) features of extra-abdominal desmoid-type fibromatoses. Methods  Plain and dynamic contrastenhanced MRI (DCE-MRI) was performed on 7 patients with extra-abdominal desmoid-type fibromatoses confirmed by surgery and pathology. The imaging features including location, dimension, edge,shape, signal intensity, enhancement pattern, and the time-signal intensity curve (TIC) of the lesions were analyzed. Results  On MR images, six lesions were infiltrative, while one was expansive. All lesions exhibited heterogeneity, with isointensity on T1WI and slight hyperintensity on T2WI. In all lesions, cordlike or patch-like low signal could be found. All lesions showed heterogeneous enhancement, and the time-signal intensity curves of the enhanced portions within the lesion demonstrated continuous ascending trend. Conclusion  Plain and DCE-MRI can characterize the imaging features as the shape, signal and enhancement pattern of extra-abdominal desmoid-type fibromatoses, which may have potential role in differential diagnosis.

    The comparison study of the blood supply of hepatic VX2 tumor by contrast-enhanced ultrasonography through the peripheral vein and portal vein
    XIAO Jing, LI Jie, WEI Yun, SHI Dandan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  131-134. 
    Abstract ( 1049 )   PDF (796KB) ( 257 )   Save
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    Objective  To evaluate the blood supply of hepatic tumor by contrast-enhanced ultrasonographythrough peripheral and portal veins. Methods  An in vivo model was studied using SonoVueTM and low mechanical index (MI) real-time gray-scale contrast-enhanced ultrasonography. SonoVueTM was rapidly injected into 12 New Zealand white rabbits with hepatic VX2 tumor through the marginal ear vein and the main trunk of the portal vein respectively. The characteristic of blood supply and enhancement pattern were analyzed. Results  SonoVueTM was administrated into 12 rabbits with 12 tumors through the peripheral vein: the tumors showed hyperechoic enhancement in the arterial phase and hypoechoic enhancement in the postarterial phase. Ultrasonographic portography was perfomed in 12 rabbits with 22 tumors: seven tumors enhanced diffusely; three tumors showed the branches of the portal vein; five tumors showed peripheral irregular enhancement; seven tumors showed no obvious enhancement. Conclusion  Contrast-enhanced ultrasonography through the peripheral vein and portal vein can manifest the blood supply of hepatic tumor, so it may be useful in the qualitative diagnosis and individual treatment of hepatic tumor.

    The differences of brain iron concentration between left- and right-handers: MRI preliminary study
    LIU Yubo1,2, WANG Guangbin2, WANG Qiangxiu3, GENG Mingbin4, WANG Lijuan1, HU Jianbin1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  135. 
    Abstract ( 1063 )   PDF (518KB) ( 440 )   Save
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    Objective  To investigate the brain iron concentration difference between left- and right-handers by phase values,  detected from susceptibility weighted corrected phase imaging at 3.0 T MR. Methods  Susceptibility-weighted corrected phase images were acquired on a 3.0T MR in 60 healthy handedness subjects (30 lefthanders and 30 right-handers) and 36 healthy subjects (more than 30 years old; 17 women, 19 men; mean age, 56.4 years old) . The left and right handedness groups were matched by sex and age (14 women, 16 men; mean age, 32.5 and 33.4 years). The phase values of bilateral frontal white matters (FWM), caudate nucleus (CN), Putamen (PU), globus pallidus (GP), thalamus (TH), red nucleus (PN) and substantia nigra (SN) were respectively measured on corrected phase images. To check on the reliability of detection method, using pearson correlation analysis to test the correlation between the corrected phase values measured from 36 volunteers with published brain iron levels. Using Independent-Sample T Test to separately (L and R handedness groups) conduct for the phase values of each of the seven bilateral brain regions. Results  Regional corrected phase values on phase-corrected images were negatively correlated with reference values of regional brain iron concentration in healthy adults(r =-0.924,P=0.003). there were no statistically significant differences in the average phase values of corresponding brain regions between left-handers and right-handers(P>0.05). Conclusion  There is no statistically significant difference of brain iron concentration between left- and right-handers.

    Diagnostic value of MR T2-mapping in early knee osteoarthritis
    YANG Jinyong1, ZHAO Bin1, YU Taifei1, MA Yanhong1, HAO Wen2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  139. 
    Abstract ( 1262 )   PDF (569KB) ( 446 )   Save
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    Objective  To measure T2 relaxation time of knee cartilage in patients with early knee osteoarthritis (OA) compared with healthy subjects of the same age group,and to evaluate the diagnostic value of the T2-mapping imaging in early knee osteoarthritis. Methods  Quantitative T2-mapping of knee cartilage was evaluated with a 3.0T MR imaging spectrometer in 20 healthy subjects and 32 patients of mild OA.,T2 values in four compartments of knee cartilage (condylus medialis and condylus lateralis femoris,condylus medialis and condylus lateralis tibiae) were measured in T2-mapping imaging. Statistical differences were analyzed. Results  The average T2 relaxation in normal group and mild OA group was (37.85±3.28)ms and (48.16±5.12)ms respectively. There was a significant difference in T2 value between case group and control group(P<0.05). Conclusion  T2-mapping imaging can indicate component abnormality in articular cartilage,and has a high clinical value in early diagnosis and surveillance of OA.

    Association between stathmin and laryngeal papilloma
    HUANG Fang1, ZHU JinZhi1,HUANG Jianqiang1, WU Chunlin2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  142-146. 
    Abstract ( 1135 )   PDF (744KB) ( 258 )   Save
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    Objective  To detect the expression of Stathmin in laryngeal papilloma(LP) and polyp of vocal cord and to explore its role in the development of LP. Methods  Immunohistochemical (PV-9000 two-step method) method was used to analyze the protein expression of Stathmin in paraffin embedded tissues from 60 cases of laryngeal papilloma and 15 cases of vocal cord polyp. Image-ProPlus software was used to determine the average optical density (MOD) of immunohistochemical image,and the relation between MOD and clinical feature of LP was analyzed. Results  The positive expression rate of Stathmin protein in LP tissues ( 88.33%) was higher than in polyp of vocal cord(53.33%), and the difference was significant(P<0.05). The expression of Stathmin protein had no relations with clinical type, disease relapse, sex and age of patients (P>0.05). Conclusion  The high expression of Stathmin protein in LP tissues may be closely related to the occurrence and development of human LP.

    Impact of forgiveness intervention on expression of anger and anger control ability of college students
    CHEN Cui1, 2, ZHANG Hongjing2, JIANG Jinghua1, WU Xiqing1, ZHANG Lina1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  147. 
    Abstract ( 1039 )   PDF (329KB) ( 396 )   Save
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    Objective  To check the impact of the forgiveness intervention on the experience of anger and anger control of college students. Method  sAccording to the forgiveness level of 225 subjects checked by the Transgression-Related Interpersonal Motivations Inventory-18, we extracted 26 subjects from them whose forgiveness levels were one standard deviation lower than the mean. Then they were randomly allocated into two groups as the experimental group and the control group and recalled their anger experiences.  HR and the state anger were used to reflect the level of the anger experience. The anger control invention was used to check the anger control ability of the subjects. The benefit-finding and empathy technique was used as the forgiveness intervention to see its impact on the expression of anger and anger control of the subjects. Results  The HR of the individuals in both the experimental group and the control group were higher when they recall their anger experience than that when recalling their relax condition, and the HR of the experimental group were lower than that of the control group when they caught the forgiveness intervention(P=0.026), as well as the state anger(P=0.048). There was no difference in the anger control ability between the experimental group and the control group(P=0.075). Conclusion  The benefit-finding and empathy technique is well as a forgive intervention which could reduce the expression of anger. Some more studies are needed to check its impact on the anger control ability of the college students.

    Effect of the medical master students′ cognitive emotion regulation strategies on depression and anxiety
    YAO Fangmin1, SONG Dongfeng2, FU Wenqing1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  150-153. 
    Abstract ( 1264 )   PDF (344KB) ( 322 )   Save
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    Objective  To explore the effect of medical masters′ cognitive emotion regulation strategies on depression and anxiety.   Methods  388 medical masters in Soochow university and  Xiamen university completed the CERQ and the depression and anxiety subscales of SCL-90. Results   ① The cognitive regulation strategies which were used more often were putting into perspective, self-blame, positive refocusing and rumination. ② The score of perspective of male students was significantly lower than that of female students (12.61±2.19/13.19±2.16, P=0.01)while the subscale scores of acceptance(10.50±2.79/9.88±2.41,P=0.02), catastrophizing (9.10±2.81/8.53±2.34, P=0.04),  and blaming others(10.92±2.54/10.31±2.28, P=0.01) were reversed.  ③ The subscale scores of rumination,  self blame and catastrophizing were significantly correlated with gender scores of  depression and anxiety(β>0.03,P<0.05).  Conclusion  The master students cognitive emotion regulation strategies have great influence on depression and anxiety, and cognitive emotion regulations of rumination, selfblames and catastrophizing can be used to forecast and explain depression and anxiety.

    Inhibitory effects of ebselen on hepatic encephalopathy of rats
    RONG Lin1, SHAO Lihua1,CHI Defeng1, WANG Shue1, WANG Xifeng1, WANG Xinting2, MA Lixian3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  154. 
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    Objective  To investigate the inhibitory role of ebselen on hepatic encephalopathy (HE) of rats and explore the probable mechanism. Methods  40 male Wistar rats were divided into 4 groups randomly: blank control group (Ⅰ), HE model group (Ⅱ), ebselen treated group (Ⅲ) and solvent control group (Ⅳ). All the groups were treated with thioacetamide (TAA) by intraper-itoneal injection except group Ⅰ (treated with normal saline) to manufacture the model of HE. Then the 4 groups were intragastrically administered with normal saline (Ⅰ, Ⅱ), ebselen (Ⅲ) and dimethyl sulfoxide (DMSO) (Ⅳ) respectively for 1 week untill the symptoms of HE changed. The rats were sacrificed to collect blood for the following detection of 3-nitrotyrosine, alanine transarninase, malondialdehyde and superoxide dismutase. Pathological sections were made to observe the liver changes. Results  The development of HE was slower in group Ⅲ  than in groups Ⅱ and Ⅳ; and the differences of serum markers between groups Ⅲ and Ⅱ, and between groups Ⅲ and Ⅳ were significant (P<0.05), while the difference between groups Ⅲ andⅠwere not obvious (P>0.05). Conclusions  The results suggest that ebselen probably has inhibitory effect on HE of rats induced by TAA and can delay the development of HE. The mechanism may be related to the capability of ebselen, which can clear peroxynitrite anion and inhibit the nitration of tyrosine.

    Quality evaluation of cartilage glycosaminoglycans ocular nanoemulsion
    CAO Jianmin1, ZHANG Lei1, GUO Bin2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(5):  157-160. 
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    Objective  To evaluate the qualities of RCG ocular nanoemulsion and to determine RCG content in nanoemulsion. Methods  RCG ocular nanoemulsion was prepared and RCG content in nanoemulsion was determined with sulfate-carbazole colorimetry method at detection wavelength of 529.0 nm.The appearance, pH values and average droplet diameter of the nanoemulsion were observed and measured. Results  The RCG ocular nanoemulsion was stable and transparent, with average droplet diameter of 51 nm, and no irritation existed. The linear range was 8.024~40.120mg/L, and the regression equation was A=0.0129c+0.008(r=0.9999).The average recovery of the method was 100.2%, and RSD was 0.92%. The RCG content in nanoemulsion was 50.03g/L. Conclusion  RCG ocular nanoemulsion is stable and accordant with the eye preparation standard of Ch.P (2005). The assay is simple, rapid and accurate, and suitable to control the quality of the ocular nanoemulsion.