Abstract:

Objective  To evaluate the effect of mifepristone on proliferation and expression of CEACAM6 of human ovarian cancer cell line SKOV3 in vitro. Methods  Human ovarian cancer cell line SKOV3 was cultured with different concentrations (control group, 1×10-7, 1×10-6, 1×10-5, 5×10-5mol/L) of mifepristone(MIF) in vitro; MTT was used to examine the cell growth; and the semi-quantity of CEACAM6 mRNA and protein were detected by RT-PCR and western blot. Results  Proliferation of SKOV3 cultured with 1×10-7, 1×10-6 mol/L of MIF was not significantly lower than the control group（P＞0.05）；5×10-5mol/L of MIF was able to significantly suppress the proliferation of SKOV3（P＜0.01）; Expressions of CEACAM6 mRNA and protein of SKOV3 in the control group were higher than that of 5×10-5mol/L of the mifepristone group（P＜0.05）. Conclusion  Mifepristone could significantly suppress the proliferation of human ovarian cancer cell line SKOV3 in vitro. The action mechanisms may be associated with the suppression of expression of CEACAM6.

Key words: Mifipristone；Ovarian neoplasms；Carcinoembryonic antigen-related cell adhesion molecule 6