Abstract:

Objectives  To explore the potential values of macrophage migration inhibitory factor(MIF) in inflammation and tumor radioimaging. Methods  Recombinant mMIF was obtained through IPTG-induced pEQ30-mMIF/M15 E.coli, and anti-mMIF McAb was made with hybridoma technology whose  specificity and biological activities were identified with Western blotting, ELISA and MMI. Anti-mMIF antibodies were radioiodinated by Na125I with Iodogen method. Results  Three stable cell lines were obtained, named as 5G2D7, 5G2C7 and 5G2E3 respectively with high specificity and biological activity to MIF. The labeling rate of 125I-anti mMIF McAb was 90.40%, the radiochemical purity was 98.36%, and specific radioactivity was 21.48MBq/μg.  Conclusion  Anti-mMIF McAb was obtained and radioiodinated successfully,which may supply a new tool for further research of MIF,especially for clinical oncology and inflammation radioimaging.

Key words: Macrophage migration inhibitory factors; Monoclonal antibody; Radioisotope; Mice; Inbred BALB/c