Abstract:

Objective  To study the effects of  sedative hypnotics on the activity of aspirin esterase in vitro. Methods  The metabolic system of aspirin esterase in vitro was established in the laboratory. The separated plasma specimens were treated with the sedative hypnotics, and then the concentrations of aspirin and its metabolite (salicylic acid）in the plasma were determined by RP- HPLC. The ratio of salicylic acid (SA) and aspirin (ASA) represented the activity of aspirin esterase. Results  The effects of diazepam, midazolam and phenobarbital on the activity of aspirin esterase were  -10.71%（P＞0.05）, -27.94%（P＜0.05）and 8.83%(P＜0.05） respectively. The activity of aspirin esterase was inhibited by midazolam, while it was induced by phenobarbital. Conclusion  The interaction between sedative hypnotics and aspirin should be paid careful attention to in clinical application.

Key words: Chromatography, High performance liquid; Sedative hypnotics drugs; Aspirin