JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2010, Vol. 48 ›› Issue (4): 120-123.

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Observation of morphology of nerve fibers with a laser scanning confocal microscope after burns

WANG Yibing1，2， HE Jiantai1， FENG Yongqiang2， LI Xia3， ZHANG Rui4， ZHANG Yangde1   

  1. 1. National Hepatobiliary Enteric Surgery Research Center, Ministry of Health, Changsha 410008，China;
    2. Department of  Burns and Plastic Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;
    3. Department of  Burns ,Weihai Municipal Hospital, Weihai 264200, Shandong, China;
    4. Department of Pathology, Shandong Medical College, Jinan 250002, China
  • Received:2010-01-10 Online:2010-04-16 Published:2010-04-16

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Abstract:

Objective  To observe the morphology of nerve fibers during reinnervation after burn. Methods  Neurofilaments of wound tissue after 1,2,3,4 weeks were determined by fluorescent immunohistochemistry. The morphology of nerves was evaluated under a fluorescent microscope and laser scanning confocal microscope(LSCM). Results  Numbers of nerve fibers within 3 weeks were less than that of normal, while these post 3 weeks were increasing. Nerve fibers got gradually longer  and were arranged parallel or like a staggeredmesh with more branches and sprouting. Uneven thickness with some swelling, distortion and local fracture were found 3 weeks after burns. Conclusion  The three dimension structure of nerve fibers can  be obtained  with a LSCM and remodeling was found during the reinnervation after burns.

Key words: Laser scanning confocal microscope; Burns; Innervation

CLC Number: 

  • R644
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