Abstract:

Objective    To construct the eukaryotic co-expression plasmid encoding interleukin-24(IL-24) and endostatin(ES), and to detect expression of the plasmid in vitro. Methods    The cDNA encoding Il-24 and ES were obtained by RT-PCR amplifications from peripheral blood and normal fetus liver respectively and cloned into an eukaryotic expression plasmid internal ribosome entry site(pIRES). The co-nstructed plasmid pIRES-IL-24-ES was confirmed by restriction enzymolysis and DNA sequencing. Fibroblast NIH3T3 cells were transformed by plasmid pIRES-IL-24-ES. RT-PCR and ELISA were used to identify the coexpression of IL-24 and ES in NIH3T3 cells. Results    The eukaryotic expression plasmid pIRES-IL-24-ES was  successfully constructed and the plasmid could  efficiently co-express IL-24 and ES in NIH3T3 cells. Conclusion    The successful construction of eukaryotic expression plasmid pIRES-IL-24-ES establishes an experimental basis for cancer treatment.

Key words: Interleukin-24； Endostatin； Expression vector； Carcinoma