Abstract:

Objective    To observe the effect of the combination of Niaoduqing on renal fibrosis of chronic renal failure rats. Methods    A total of 36 rats were allocated into 3 groups: the normal control group, the operation control group and the Niaoduqing group. The normal control group was given physiological saline and the other 2 groups were established by adriamycin and unilateral nephrectomy. The operation  group and the normal control group were given normal saline. The Niaoduqing group were given Niaoduqing for 8 weeks. At 4 weeks and 8 weeks， the 24-hour urinary protein excretion and renal function were observed. At 8 weeks there was pathological change of nephridial tissue, and immunohistochemistry was assayed to measure the expressions of α-SMA and collagenIV. Results    Higher expressions of α-SMA,COLIV and more severe glomerulosclerosis in the kidney(P＜0.01),　as well as more loss of renal function were observed in the operation group than  in the normal control group(P＜0.01). Those changes were less severe in the Niaoduqing group than in the operation  group(P＜0.01), suggesting Niaoduqing could significantly decrease expressions of α- SMA and COLIV, and also could slow down the loss of renal function. Conclusion    Niaoduqing could significantly reduce urinary protein excretion,  improve renal function, inhibit epithelial cell phenotypic change of renal tubules, and delay renal fibrosis.

Key words: Renal fibrosis; Niaoduqing; α-Smooth muscle actin； Collagen Ⅳ