Abstract:

Objective     To investigate the effects of APS on TLR4、 NF-κB expression and signal pathway in vascular endothelial cells injured by hypertension patients′ blood. Methods     The human umbilical vein endothelial cells  (HUVEC-Cs) were intervened by 10% serum of  hypertension patient or healthy human for 24h.  TLR4mRNA expression in HUVEC-Cs was detected by real time PCR. After the lipopolysaccharide(LPS) activated HUVEC-Cs were effected by different dosage APS, TLR4 and NFκB mRNA were detected by real time PCR, TLR4 and NF-κB protein were detected by western blot. Results    After the serum acted the HUVEC-Cs for 24h, TLR4mRNA expression was increased in hypertension disease group than the healthy group(P<0.01). APS showed the ability of reducing TLR4mRNA expression, inhibiting TLR4 protein expression and suppressing  IκB protein degeneration in LPS activated HUVEC-Cs in a dose-dependent manner(P<0.05, P<0.01, P<0.001).  Conclusion    Inflammatory reaction and immunity disorder initiated by TLR-NF-κB signal pathway is one of the influencing mechanisms in the damage of vascular endothelial cells of hypertension disease. APS can reduce the damage.

Key words: Essential hypertension; Vascular endothelial cells; Toll-like receptor 4; Nuclear factorκB; Astragaluspolysaccharide