JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2010, Vol. 48 ›› Issue (12): 124-.

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Effect of Cystatin C on Cathepsin S and extracellular matrix  in human arterial smooth muscle cells

WANG Qun1, DU Yi-meng2, DONG Zhao-qiang2, LIU Yu-sheng2, LU Qing-hua2   

  1. 1. Hospital of South Division, Shandong University, Jinan 250061, China；2.Department of Cardiovascular Diseases, The Second Hospital of Shandong University, Jinan 250033, China
  • Received:2010-10-27 Published:2010-12-16

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Abstract:

Objective     To study the effect of Cystatin C on Cathepsin S and extracellular matrix (ECM) in human arterial smooth muscle cells(ASMCs). Methods     ASMCs were divided into the control group(normal cells), the Cystatin C highly expressed group, the Cystatin C silenced group and the independent interference group. Expression of Cathepsin S was detected by RT-PCR and Western blot at different time. Contents of collagenⅠ, laminin(LN) and fibronectin(FN) were detected by ELISA in ASMCs of the control group, the Cystatin C highly expressed group(24h and 48h) and the Cystatin C silenced group(48h and 72h). Results     Expression of Cathepsin S in the Cystatin C highly expressed group was lower than in the control group, and the difference between the two groups was not statistically significant（P>0.05）. Expression of Cathepsin S in the Cystatin C silenced group was obviously strengthened with the increase of interference time(P<0.01). While at the same time, expression of Cystatin C was weakened. The result of ELISA showed that content of collagenⅠ in each group was increased or decreased, while the differences were not statistically significant（P>0.05）. Contents ofLN and FN increased in the Cystatin C highly expressed group (24h and 48h), and the differences were both statistically significant compared with the controls(P<0.05). Compared with the control group, content of LN in Cystatin C silenced group (48h) was insignificantly decreased（P>0.05）, while it was obviously decreased in Cystatin C silenced group (72h)（P<0.05）.Compared with the control group, contents of FN in Cystatin C silenced group (48h and 72h) decreased markedly, and the differences were both statistically significant（P<0.05）. Conclusion      Expression of Cathepsin S is very low in normal ASMCs. Expressions of Cystatin C and Cathepsin S in ASMCs are inversely correlated. The balance of Cystatin C and Cathepsin S has a significant effect on LN and FN to different degrees, but no significant effect on collagenⅠ.

Key words: Cystatin C; Cathepsin S; Extracellular matrix; Human arterial smooth muscle cells

CLC Number: 

  • R541.4
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